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结球甘蓝相对于大白菜的连锁群特异SSR标记建立
  • 期刊名称:园艺学报
  • 时间:0
  • 页码:1221-1226
  • 语言:中文
  • 分类:S635.1[农业科学—蔬菜学;农业科学—园艺学] S634.1[农业科学—蔬菜学;农业科学—园艺学]
  • 作者机构:[1]河北农业大学园艺学院,河北保定071000
  • 相关基金:国家自然科学基金项目(30871713);河北省自然科学基金项目(C2005000222);河北农业大学将帅计划项目
  • 相关项目:大白菜—结球甘蓝异附加系的合成及细胞分子遗传研究
中文摘要:

选用位于甘蓝9个连锁群的91对SSR引物,对8个结球甘蓝品种和7个大白菜品种进行PCR扩增,筛选出25对结球甘蓝相对大白菜的特异SSR引物,涉及甘蓝的9个连锁群,其中位于O1连锁群3对,O2连锁群4对,O3连锁群2对,O4连锁群3对,O5连锁群2对,O6连锁群2对,O7连锁群4对,O8连锁群2对,O9连锁群3对。为利用SSR标记鉴定大白菜—结球甘蓝异附加系中附加的结球甘蓝染色体奠定了基础。

英文摘要:

Ninety-one primers from 9 cabbage linkage groups were amplified on 8 cabbage cultivars and 7 Chinese cabbage cultivars, 25 of them were specific on cabbage compared with Chinese cabbage. These primers assigned 9 linkage groups of cabbage, 3 of them were assigned linkage group O1, 4 of them were assigned linkage group 02, 2 of them were assigned linkage group 03, 3 of them were assigned linkage group 04, 2 of them were assigned linkage group 05, 2 of them were assigned linkage group 06, 4 of them were assigned linkage group 07, 2 of them were assigned linkage group 08, and 3 of them were assigned linkage group 09. It laid a foundation to further identify the introduced chromosome of cabbage in Chinese cabbage alien addition lines.

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