中文摘要：

目的：建立杜仲多指标HPLC指纹图谱,并应用一测多评法对药材中松脂醇二葡萄糖苷（PDG）、绿原酸（CA）、京尼平苷酸（PA）的含量同时进行测定。方法：采用WelchromTMC18110A（4.6 mm×250 mm,5μm）色谱柱,以甲醇-0.3%冰醋酸进行梯度洗脱,柱温20℃,流速1 mL·min-1,检测波长238 nm,以松脂醇二葡萄糖苷、绿原酸、京尼平苷酸和京尼平苷（GP）为指标性成分建立杜仲药材的指纹图谱。以绿原酸为参照内标,通过相对校正因子对松脂醇二葡萄糖苷、绿原酸、京尼平苷酸进行含量测定。结果：建立了杜仲多指标HPLC指纹图谱,标定了12个共有指纹峰。对杜仲中3个成分用一测多评法进行了测定,其计算值与外标法实测值之间没有明显差异。结论：建立了杜仲药材多指标HPLC指纹图谱和一测多评法对松脂醇二葡萄糖苷、绿原酸、京尼平甘酸进行同时测定的方法,该方法简便可行,为更全面合理科学地对杜仲药材质量的评价提供了技术支撑。

英文摘要：

Objective： To establish the multi-indicator HPLC fingerprint of Eucommiae Cortex and to determine the contents of geniposidic acid, chlorogenic and acid, pinoresinol diglucoside with quantitative analysis of multi-components by single marker. Method： Welchrom ^TMC18 110A （4.6 mm×250 mm, 5 μm） column was used, the mobile phase was consisted of methanol-water-0. 3% acetic acid as gradient elution. The column temperature was at 20℃. The flow rate was 1.0 mL .min-1 and the fingerprint was detected at 238 nm. Geniposidic acid and chlorogenic acid, pinoresinol diglucoside and geniposide was choosen as marks of ingredients to establish HPLC fingerprint. Using chlorogenic acid＇ as internal reference standard, the contents of geniposidic acid, chlorogenic acid, pinoresinol diglucoside was determined according to the relative correction factor. Result： All tested samples contained the 12 common peaks and there was no significant differences between the quantitative results of three ingredients of quantitative analysis of multi-components by single marker method and external standard method. Conclusion： The muhi-indicator HPLC fingerprint and simultaneous determination of geniposidic acid and chlorogenic acid, pinoresinol diglucoside with quantitative analysis of multi-components by single marker were firstly studied. This method was simple and feasible, which would provide better technique support for the quality control of Eucommiae Cortex.