中文摘要：

目的探讨前列腺癌相关长链非编码RNA（prostate cancer relatedlong non—coding RNA，PCRI。）在前列腺癌中的表达情况及其潜在的生物学功能。方法采用qRT—PCR检测PCRI。在前列腺癌组织、癌旁组织及其他组织中的表达，同法检测并比较PCRL在雄激素依赖（I．NCaP—AD）与雄激素非依赖（LNCaP—AI）前列腺癌细胞和正常前列腺上皮细胞（RWPE-1）中的表达差异。以siRNA干扰PCRL后，采用qRT—PCR方法检测LNCaP—AI细胞和LNCaPAD细胞中雄激素受体的表达变化。结果PCRL在前列腺及前列腺癌组织中特异高表达，LNCaP—AI细胞中PCRL水平高于LNCaP—AD细胞和RWPE一1细胞。干扰PCRL后LNCaPAI细胞和LNCaP—AD细胞中雄激素受体的表达量上升（前者P〈0．0001）。结论在前列腺癌中特异高表达的PCRL与前列腺癌的进展有关，PCRI。和雄激素受体之间可能存在一定的调控关系。

英文摘要：

Objective To investigate the expression of prostate cancer related long non-coding RNAs （PCRI,） in prostate cancer and its biological function. Methods The expression levels of PCRL in prostatic cancer tissues, adjacent tissues and tissues of other organs were examined by qRT PCR. The expression levels of PCRL in androgewdependent （LNCaP-AD）, androgen-independent （LNCaP AI） prostatic cancer cell lines and normal prostate epithelial cells （RWPE-1） were also compared by using qRT-PCR. Finally, the expression levels of androgen receptor were compared between LNCaP-AD and I,NCaP-AI prostatic cancer cells by qRT PCR after siRNA interference of PCRL expression. Results PCRL were highly expressed in the prostate and prostatic cancer tissues. Expression of PCRL was notably higher in LNCaP-AI cells than in LNCaP-AD and RWPE-1 cells. Interference of PCRL expression in LNCaP-AI and I,NCaP AD cells significantly increased androgen receptor level （P~0. 000 1 for LNCaP-AI cells）. Conclusion Overexpression of PCRL in prostatic cancer is related to the progression of prostatic cancer; and regulatory relation may exist between PCRL and androgen receptor.