从福建平潭岛不同生境采集的野生坛紫菜,经过2~3a的筛选和培育获得野生品系b,c和d,通过体细胞酶解和自繁,获得丝状体和叶状体。实验结果表明:(1)野生选育品系b,c,d生长较快,日生长达3.25~3.75cm/d,未选育的品系a日生长为2.12cm/d,传统的养殖品种生长速度慢,日生长仅有1.37cm/d;(2)选育的品系b对高温具有明显的耐受性,在高温30℃培养10d,日平均增重率达4.34%,而对照组在水温27℃时培养5d以上,藻体就开始腐烂;(3)品系b耐低氮、磷能力较强,在低氮、磷培养条件下,第9天色泽变为浅黄色,但没有腐烂死亡,此时将藻体移至含有氮、磷的正常培养液下培养3d,藻体就可恢复正常,对照组的藻体在相同条件下,第6天藻体中部就开始糜烂,第9天叶片就有2/3溃烂;(4)在品质方面,品系b的总藻胆蛋白含量高达98.89mg/g,对照组的总藻胆蛋白含量仅有50.04mg/g;本研究结果可为坛紫菜的遗传改良、野生品系选育及利用奠定基础。
The samples of wild Porphyra haitanensis were collected from different environment in Pingtan Island of Fujian Province. Through enzymolysis to somatic cells and their self-reproduction, the conchocelis and thallus of wild stain B, C and D were obtained after 2-3 years'selection and cultivation. The results are as follows: (1) The wild-selected strain B, C and D exhibited relatively faster growth, which was about 3.25-3.75 cm/d. The thallus growth of Strain A, which has not been selected, was about 2.12 cm/d. Traditional cultivated strain exhibited the slowest growth, only 1.37 cm/d. (2)Strain B exhibited remarkable resistance to high temperature. The increasing rate of weight per day could reach up to 4.34% after cultured for 10 days in 30 ℃, while the control stain began to rot after cultured for 5 days in 27 ℃. (3) Strain B exhibited remarkable tolerance to low capacity of N and P. In low nitrogen and phosphorus environments, the blade's color of strain B became buff after 9 days'cultivation. But it recovered to normal condition after cultured for 3 days in seawater of normal content of nitrogen and phosphorus. However, the control group began to rot in the middle part of blade after 6 days and the rotten proportion was about 2/3 in 9thdays in the same condition. (4) The phycobiliproteinin content of stain B was 98.89 mg/g, but the number was only 50.04 mg/g when it comes to control group. Therefore, strain B shows superiority in growth rate, high-temperature resistance and low-N-and-P tolerance. The results are helpful for genetic improvement, wild strains'selective breeding and utilization in P. haitanensis.