欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
建立了疏水性离子液体1-丁基-3-三甲基硅咪唑六氟磷酸盐（BtmsimPF。）萃取细胞色素c（Cyt—e）的新方法。在萃取条件为pH1．0，Cyt—c溶液体积3mL，BtmsimPF6体积400μL，萃取时间30min时，5mg／LCyt—c的萃取效率为85％。萃取机理研究表明：在酸性条件下，Cyt—c发生构型转变，肽链伸展，使疏水性基团外露，增加了Cyt—e在疏水性离子液体中的溶解性；同时，当pH〈2．0时，血红素基团中的Fe与Met-80的配位断裂，空出第六个配位空间，离子液体咪唑阳离子进入到肽链孔穴与Fe发生配位，形成新的配位键，从而被萃取到离子液体中。
英文摘要：
A novel method was developed for the extraction of cytochrome c （Cyt-c） from aqueous solution into ionic liquid 1-butyl-3-trimethylsilylimidazolium hexafluorophosphate （Btmsim PF6 ）. An extraction efficiency of ca. 85% was achieved for 5.0 mg/L Cyt-c, by extracting 3 mL of Cyt-c aqueous solution with 400 μL of Btmsim PF6 for 30 min at pH 1.0. The extraction mechanisms were investigated by UV-Vis and circular dichroism spectra. It was demonstrated that conformation change of Cyt-c was encountered in an acidic medium （pH 〈 5 ） accompanied by the unfolding of peptide chain and the exposure of hydrophobic groups of berne, which results in the dissolution of Cyt-c in ionic liquid Btmsim PF6. Meanwhile, a cleavage of the 6th coordination bond of the iron atom occurred at pH 1.0 by releasing the Met-80 ligand and giving rise to a vacant coordination position. Thus, the covalent coordination between the cationic Btmsim ~ of ionic liquid and the iron atom of heme group facilitates the transfer of Cyt-c into ionic liquid Btmsim PF6.
同期刊论文项目
同项目期刊论文
Systematic characterization of the covalent interactions between epigallocatechin gallate and peptid
The miniaturization of bioanalytical assays and sample pretreatments by exploiting meso-fluidic lab-
Selective isolation of acidic proteins with a thin layer of multi-walled carbon nanotubes functional
One system with two fluorescence resonance energy transfer (FRET) assembles among quantum dots, gold
Fiber-optic fluorescence sensor for dissolved oxygen detection based on fluorinated xerogel immobili
Improved selection of LMW over HMW proteins from human plasma by mesoporous silica particles with ex
Isolation of hemoglobin from human blood by solid phase extraction with lanthanum(III) modified zeol
A pH-responsive poly(N-isopropylacrylamide -co-acrylic acid) hydrogel for the selective isolation of
Poly(phthalazine ether sulfone ketone) as novel stationary phase for stir bar sorptive extraction of
Biological cell-sorption for separation/preconcentration of ultra-trace cadmium in a sequential inje
New developments in flow injection/ sequential injection on-line separation and preconcentration cou
Selective extraction of low molecular weight proteins by mesoporous silica particles with modified i
Functionalization of multiwalled carbon nanotubes with hyperbranched polyethyleneimine for highly se
A highly fluorescent hydrophilic ionic liquid as potential probe for the sensing of biomacromolecule
A new poly (phthalazine ether sulfone ketone)-coated fiber for solid-phase microextraction to determ
Surface-assembly of graphene oxide nanosheets on SiO2 particles for the selective isolation of hemog
Combination of Improved 18O Incorporation and Multiple Reaction Monitoring: A Universal Strategy for
Coating a thin layer of NaBH4 solution for mercury vapor generation coupled to atomic fluorescence s
Direct and indirect immunolabelling of HeLa cells with quantum dots Direct and indirect immunolabell
Speciation of inorganic arsenic in a sequential injection dual mini-column system coupled with hydri
Arsenic speciation with gradient hydride generation interfacing liquid chromatography and atomic abs
Arsenic preconcentration via solid phase extraction and speciation by HPLC-gradient hydride generati
A radial microfluidic concentration gradient generator with high-density channels for cell apoptosis
Multistage Coloring Electrochromic Device Based on TiO2 Nanotube Arrays Modifi ed with WO 3 Nanopart
A Highly Fluorescent Hydrophilic Ionic Liquid as a Potential Probe for the Sensing of Biomacromolecu
Chitosan/carbon nanotube composites for the isolation of hemoglobin in the presence of abundant prot
Atmospheric pressure dielectric barrier discharge as radiation source for optical emission spectrome
Selective extraction/isolation of hemoglobin with ionic liquid 1-butyl-3-trimethylsilylimidazolium h
Isolation and pre-concentration of basic proteins in aqueous mixture via solid-phase extraction with
Selective isolation of acidic proteins with a thin layer of multiwalled carbon nanotubes functionali
Silk fibroin as a sorbent for on-line extraction and preconcentration of copper with detection by el
Isolation of hemoglobin from human blood using solid phase extraction with lanthanum(III) modified z
Precipitate coating on cellulose fibre as sorption medium for selenium preconcentration and speciati
期刊信息
位置：