中文摘要：

将大豆中已克隆的一个新的SKIP基因（GmGBP1）构建到原核表达载体pGEX-6p-1上,导入大肠杆菌BL21（DE3）中,对其进行IPTG诱导。结果表明：在IPTG浓度为0.1 mmol.L-1,诱导时间为8 h,诱导温度为37℃时,重组蛋白得到表达,分子量大约为95 kDa,SDS-PAGE电泳结果表明重组蛋白主要以包涵体形式存在,用8 mol.L-1尿素对其进行溶解后经GST柱纯化,得到较好的纯化效果。

英文摘要：

A new SKIP homolog in soybean was constructed into prokaryotic expression vector pGEX-6p-1 and then transformed into E.coli BL21（DE3）to get the expression protein for further study.The result indicated that an 95 kDa recombinant protein was expressed with the treatment of 0.1 mmol·L-1IPTG for 8 h at 37℃,the recombinant protein was confirmed to mainly existed in inclusion body form by SDS-PAGE.The high-quality recombinant protein was obtained through GST column purification after the inclusion body was dissolved with 8 mol·L-1 urea.