中文摘要：

在猪输卵管上皮细胞（pOECs）与猪卵巢皮质细胞（pOCCs）共培养体系中进行猪卵子体外成熟（IVM）,研究比较了pOECs与pOCCs对猪卵母细胞体外成熟（IVM）、体外受精与胚胎发育的影响。结果显示：pOCCs或pOECs参与猪卵子体外成熟可显著提高卵丘扩散与MⅡ期卵子的比例（P〈0.05）,然而GV期卵子的比例却没有显著减少（P〉0.05）;pOCCs组内成熟卵子的GSH含量显著高于其余各组（P〈0.05）,而GSH在pOECs组与对照组之间差异不显著（P〉0.05）;尽管pOCCs和pOECs共成熟可显著降低多精入卵的比例（P〈0.05）,但是受精率与雄原核形成率（MPN）在各组间却无显著差异（P〉0.05）;pOCCs和pOECs共培养均可以显著提高卵裂率与囊胚发育率（P〉0.05）,然而囊胚细胞数在处理组与对照组间没有显著性差异（P〉0.05）。研究认为,pOCCs与pOECs共培养体系能显著提高猪卵母细胞IVM质量,降低多精入卵的发生,并提高囊胚发育率。

英文摘要：

Porcine oocytes were co-cultured respectively with porcine ovarian cortex cells（pOCCs） and porcine oviductal epithelial cells（pOECs） during in vitro maturation（IVM）.The effects of pOCCs and pOECs co-culture during IVM on the maturation,fertilization and embryos development in vitro were studied.The results showed that cumulus significantly expanded and matured and MⅡoocytes in the co-culture groups were higher than that in the control group（P＆lt;0.05）,whereas there was no significant difference in unmatured GV oocytes between the groups（P＆gt;0.05）.In addition,the GSH concentration in the pOCCs group was higher than that in other groups（P＆lt;0.05）.After IVF,polyspermy in the co-culture groups were lower than that in the control group（P＆lt;0.05）,and there were no significant difference in both fertility and male pronucleus（MPN） formation between the groups（P＆gt;0.05）.In conclusion,the presence of the pOCCs and pOECs during IVM enhanced the maturation quality of the porcine oocytes,reduced the polyspermy,and increased the percentages of blastocyst.