中文摘要：

目的：探讨解毒三根汤（虎杖根、水杨梅根、藤梨根）对肿瘤相关成纤维细胞（CAFs）的血管内皮生长因子（VEGF）、白介素-6（IL-6）的影响以及对Caspase信号通路的探讨。方法：BALB/c小鼠皮下接种CT-26结肠癌细胞（1.2×10^6m L）;随机分为3组：正常对照组、模型组、解毒三根汤组,ELISA检测3组CAFs上清液中VEGF、IL-6的表达量,实时荧光定量PCR检测各组CAFs中Caspase-3、Caspase-8的m RNA表达量。结果：24h及48h解毒三根汤组刺激的CAFs上清液中VEGF、IL-6的表达量较模型组显著降低（P〈0.01）;解毒三根汤组Caspase-3、Caspase-8 m RNA表达量较模型组明显上升（P〈0.01）。结论：解毒三根汤降低CAFs上清液中VEGF、IL-6的表达量,提高CAFs中Caspase-3、Caspase-8 m RNA的表达量,可能是其抑制肿瘤细胞迁移侵袭的机制。

英文摘要：

Objective： To observe the effect of Jiedu Sangen Decoction （JSD, consisting of Polygonum cuspidatum, Geum japonicum thumnb, Radix actinidiae） on the expression of carcinoma-associated fibroblasts （CAFs） such as vascular endothelial growth factor （VEGF）, IL-6 and caspase signal pathway. Methods： The BALB/c mice were subcutaneously inoculated with colon cancer CT-26 cells （1.2×10^6mL） and then randomly divided into 3 groups, a normal control group, a model group, a JSD group. The expression of VEGF and IL-6 in the supernatant of CAFs was detected by using ELISA. The mRNA expression of caspase-3 and caspase-8 was detected by real-time fluorescence quantitative PCR. Results： Compared with the model group, the expression of VEGF and IL-6 in the supernatant of CAFs decreased in the JSD group at 24h and 48h （P〈0.05, P〈0.01）. The mRNA expression of caspase-3 and caspase-8 in the JSD group increased obviously compared with the model group, and the difference was statistically significant （P〈0.01）. Conclusion： JSD could decrease the expression of VEGF and IL-6 in the supernatant of CAFs, and increase the mRNA expression of caspase-3 and caspase-8. This might be possible mechanisms for inhibiting the migration and invasion of tumor cells.