中文摘要：

壳寡糖可以抑制多种病原微生物的生长．其抑菌活性与其数均相对分子质量有密切关系．由专一性的壳聚糖混合酶、内切酶和外切酶降解制备3种不同数均相对分子质量的壳寡糖C-1、C-2和C-3样品，通过抑制生长速率的方法，研究了3种壳寡糖对几种常见植物病原菌的拮抗作用，并采用抑菌圈法测定了壳寡糖对植物病原菌的最低抑菌质量浓度．结果表明，C-1和C-2都有较好的抑菌活性，C-2的抑菌活性明显优于C-1，C-3基本上没有抑菌活性，反而促进了植物病原菌的生长．抑菌效果因壳聚糖酶的酶切方式、壳寡糖的质量浓度和供试菌种而异．

英文摘要：

Chitooligosaeeharides ean inhibit many mieroorganisms, with its antimierobial aetivity being related to its molecular weight. The chitooligosaceharides C-1, C-2 and C-3 were prepared respectively by chitosanase, endo-chitosanase and exo-chitosanase. The antimicrobial activity of three chitooligosaccharides with different molecular weights were examined against seven pohytopathogenic fungi by determining the inhibitory growth curves. The minimum inhibitory concentration of ehitooligosaeeharides C-1 and C-2 were examined by determining inhibitory circles. C-1 showed higher antimicrobial activity than C-2, C-3 had no antimicrobial activity nearly, and to some extent, C-3 promoted the growth of some pohytopathogenie fungi. The effect of antimierobial activity depends on species of enzymolysis, concentration of ehitooligosaecharide and categories of pohytopathogenie fungi.