中文摘要：

目的应用定量聚合酶链反应（PCR）方法测量端粒长度。方法随机选取63例健康人外周血样本，采用定量PCR方法测量端粒长度相对T／S比率、DNA印迹法测定端粒限制性片段（TRF）长度并进行相关性分析。结果定量PCR测量端粒长度相对T／S比率为0．76±0．27，DNA印迹法测量平均TRF值为9．20±1．12，两种方法测量的结果相关性分析R^2=0．575，P〈0.01。结论采用定量PCR方法测量端粒长度具有重复性好、省时、简便、可靠，可高通量的处理大量样品的特点，端粒的长短同癌症的发生有密切联系。本方法值得推广应用于癌症的遗传学和分子流行病学研究。

英文摘要：

Objective Real - time quantitative PCR has been applied for measurement of telomere length. Methods Sixty - three samples of peripheral blood from healthy individuals were randomly selected for study. The relative T/S ratio of telomere length was measured by real - time quantitative PCR technique. The terminal restriction fragment （TRF） length of telomere DNA was measured by Southern blotting. The correlation between result of relative T/S ratio and mean TRF length was analyzed. Results The mean relative T/S ratio of telomere length was 0.76 ± 0.27 and the mean TRF length was 9.20 ± 1.12. The value of relative T/S ratio of telomere length was positively correlated with mean TRF length （ R^2 = 0. 575, P 〈 0.01 ）. Conclusion Real - time quantitative PCR has advantages of simplicity and rapidity and it is readily scalable to achieve a high throughput of samples. This method will facilitate the investigations on biology of telomeres and it plays an important role in study of molecular pathogenesis of cancer.