中文摘要：

以三联吡啶钌（Ru（bpy）3）为内核材料,通过反相微乳液法合成了表面带氨基的核壳结构荧光纳米粒子Ru（bpy）3/SiO2,利用透射电子显微镜、荧光光谱、紫外-可见光谱等手段进行表征,并进行了光稳定性、荧光分子泄露与纳米粒子表面氨基测定等实验,结果表明：所合成的纳米粒子表面带氨基活性基团,每毫克纳米粒子约含385nmol氨基,纳米粒子呈规则球形,大小均一,单分散性好,平均粒径为（70±6）nm,具有很好的光稳定性。用100W氙灯在最大发射波长照射90min后,其荧光强度仅衰减8%;在水溶液中不易发生染料泄露,连续超声1h后,染料泄露少于0.05%。以合成的纳米粒子作荧光探针标记链霉亲和素后应用于蛋白质微阵列芯片检测HIV p24抗原。结果显示,荧光强度与p24浓度呈良好的正相关性,检出限为3.1μg/L。本纳米粒子作为新型荧光探针,可应用于高灵敏检测的蛋白质微阵列芯片及荧光免疫分析等系统。

英文摘要：

A core-shell type of tris（2,2′-bipyridyl）ruthenium（Ru（bpy）3）-doped silica fluorescent nanoparticles with amino groups on the surface was developed by a water-in-oil microemulsion method. The characteri-zations by transmission electron microscopy （TEM）,UV-Visible spectra and fluorescent spectra show that the nanoparticles are amino groups functioned （385 nmol amino groups per milligram nanoparticles）,spherical,monodisperse,and uniform in size with the diameters being （70±6） nm. Due to a lot of Ru（bpy）3 molecules encapsulated in the silica matrix that also severs to protect Ru（bpy）3 dye from photodamaging oxidation,the nanoparticles are extremely bright,photo stable and chemical stable. The fluorescent intensity of the pure Ru（bpy）3 was decreased approximately 56% after 90 min of continuous excitation with 100 W of Xenon lamp,whereas the fluorescent intensity of the nanoparticles was only decreased 8%. When the nanoparticles were continuously ultrasonic 60 min in aqueous solution,their fluorescent intensity was only decreased approximately 0.05%. The nanoparticles were conjugated with streptavidin and applied in protein microarray for detection of HIV p24. Results show that fluorescence intensity and p24 concentration present a good positive correlation. The analytical sensitivity is 3.1 μg/L. These results demonstrate the potential to apply the nanoparticles in ultrasensitive protein microarray and fluorescent immunoassay systems.