中文摘要：

目的：研究系统性硬皮病（SSc）患者外周血CD4＋T细胞中CD70的表达水平。方法：应用密度梯度离心法分离17例SSc患者（女性12例，男性5例）和13例对照者（女性8例，男性5例）的外周血单个核细胞，磁珠分选CD4＋T细胞。RT-PCR检测CD4＋T细胞中CD70mRNA的表达水平；流式细胞术检测CD70蛋白在CD4＋T细胞的表达水平。结果：与对照组相比，SSc患者CD4＋T细胞中CD70mRNA和CD70蛋白表达均明显升高（P=0．001；P=0．007）。结论：CD70在SSc的发生发展中可能起着重要作用。

英文摘要：

Objective：To investigate the expression level of CD70 in CD4＋ T cells from peripheral blood of systemic sclerosis （SSc） patients. Methods： Peripheral blood mononuclear cells were isolated from peripheral venous blood of SSc patients （12 females, 5 males） and healthy donors （8 females, 5 males） by density gradient centrifugation. CD4＋ T cells were isolated using magnetic beads. Real-time quantitative polymerase chain reaction （RT-PCR） was adopted to measure mRNA level of CD70 in CD4＋T ceils. The CD70 protein expression level on the surface of CD4 ＋T cells was analyzed by flow cytometry. Results：Expression of both CD70 mRNA and protein in CD4＋T cells was significantly elevated in PBMC from SSc patients compared with healthy controls （P =0. 001 ;P = 0. 007, respectively）. Conclusion： CD70 may play an important role in the pro-gression of SSc.