中文摘要：

目的：观察α-突触核蛋白（α-Synuclein,α-Syn）对小鼠空间学习记忆能力的影响.方法：以C57/B6野生型小鼠（Wild Type,WT）及α-Syn过表达转基因小鼠（Tg-α-Syn）为研究对象,采用Moms水迷宫（Morris water maze,MWM）中定位航行实验、空间探索实验以及可视平台实验,按照训练要求,记录两组小鼠游泳路径和完成任务的时间.结果：在定位航行试验中,随着训练时间的增加,两组小鼠的寻台潜伏期均缩短.同时,在训练第五天时,与WT组小鼠相比,Tg-α-Syn小鼠的寻台潜伏期明显延长（58.77±1.32 versus 17.34± 6.44,＊P＜0.05）.在空间探索实验中,与WT组小鼠相比,Tg-c-Syn小鼠在目标象限停留时间更短（17.91±2.14versus 5.59±4.98,＊P＜0.05）.为了进一步证明Tg-α-Syn小鼠的空间记忆与学习能力下降是与海马相关,采用了可视平台实验,结果显示：两组小鼠的寻台潜伏期无显著性差异（P＞0.05）,即Tg-α-Syn小鼠不存在运动障碍和视觉障碍.结论：α-Syn过表达可导致海马相关的空间记忆、学习能力受损.

英文摘要：

Objective：To investigate the effect of α-Synuclein on the spatial learning and memory of mice.Methods：The transgenic mice over-expressed α-Syn were used as models to observe the swimming path and the time to complete the task in the Morris water maze which included the navigation test,spatial probe test and visual platform experiment.Results：In the navigation test,the time to search for the two groups of mice was shortened with the training time prolonged.Compared with the wild type mice,the escaped latency oftransgenic mice over-expressed α-Syn was prolonged in the navigation test （58.77±1.32 versus 17.34± 6.44,＊P〈0.05） and the target quadrant shortened（17.91± 2.14 versus 5.59±4.98,＊P〈0.05）.Meanwhile,in order to further prove that the spatial memory and learning ability of Tg-α-Syn mice are related to hippocampus.There was no significant difference between the two groups of mice in the search latency of visual platform experiments （P〉0.05）.Conclusion：The over-expression ofα-Syn could impaire the spatial memory and learning depended hippocampus.