位置：成果数据库 > 期刊 > 期刊详情页
Production of the Polyclonal Anti-human Metallothionein 2A Antibody with Recombinant Protein Technology
  • ISSN号：1672-9145
  • 期刊名称：《生物化学与生物物理学报：英文版》
  • 时间：0
  • 分类：Q51[生物学—生物化学]
  • 作者机构：[1]The State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, China
  • 相关基金：This work was supported by the grants from the Ministry of Education of China （TRAP0YT 1999028418, SRFDP 20030284040）, and the National Nature Science Foundation of China （No. 30270291, 30330530, 20373026 and 30425009）
作者： Faiz M. M. T. MARIKAR Qi-Ming SUN Zi-Chun HUA[1]
关键词： 多克隆抗体, 金属硫蛋白, 抗体, 重组蛋白技术, metallothionein 2A, glutathione S-transferase, polyclonal antibody
中文摘要：

Metallothionein 2A (MT2A ) 是能被暴露导致到有毒的金属的小压力反应蛋白质。它高度在乳癌房间被表示。在这研究，编码人的 MT2A 蛋白质的 cDNA 在 Escherichia 关口 i 被表示为谷胱甘肽 S-transferase (GST ) 熔化蛋白质。Recombinant MT2A 蛋白质被装载到 12% 钠 dodecylsulfate-polyacrylamide 胶化上并且由电气泳动分开了， recombinant 蛋白质被染色的 Coomassie 蓝色和 33 kDa recombinant GST-MT2A 熔化蛋白质设想乐队从胶化被切掉。胶化片被切碎并且过去常产生 polyclonal 反时代。对 MT2A 蛋白质的兔子的免疫允许高 titer polyclonal 抗血清的生产。这新 polyclonal 抗体在西方的污点分析认出了 recombinant MT2A 蛋白质。这便宜抗体将为在各种各样的免疫分析的察觉是有用的。

英文摘要：

Metallothionein 2A （MT2A） is a small stress response protein that can be induced by exposure to toxic metals. It is highly expressed in breast cancer cells. In this study, the cDNA encoding the human MT2A protein was expressed as glutathione S-transferase （GST） fusion protein in Escherichia coli. Recombinant MT2A proteins were loaded onto 12% sodium dodecylsulfate-polyacrylamide gel and separated by electrophoresis, the recombinant protein was visualized by Coomassie blue staining and the 33 kDa recombinant GST-MT2A fusion protein band was cut out from the gel. The gel slice was minced and used to generate polyclonal antisera. Immunization of rabbit against MT2A protein allowed the production of high titer polyclonal antiserum. This new polyclonal antibody recognized recombinant MT2A protein in Western blot analysis. This low-cost antibody will be useful for detection in various immuno-assays.

同期刊论文项目
HMGA2与DNA结合的热力学研究及其与DNA超螺旋的关系
期刊论文 4
FADD磷酸化及其对细胞增殖和发育的调控作用
期刊论文 4
FADD突变小鼠免疫细胞的基因表达谱和蛋白质谱及其生物学功能
期刊论文 30 会议论文 19 获奖 1 著作 2
分子免疫学
期刊论文 33 会议论文 29 著作 1
同项目期刊论文
TDG蛋白的表达纯化及多克隆抗体的制备
Expression of Recombinant Human FADD, Preparation of Its Polyclonal Antiserum and the Application in Immunoassays
Large scale preparation of the
利用荧光标记引物和DNA自动测序仪确定DNA的断裂位点
Expression, Purification and Characterization of C-FADD
FAK Expression Regulation and Therapeutic Potential.
Enhancement of the antitumor properties of TRAIL by its targeted delivery to the tumor neovasculatur
Immobilized protein ZZ, an Affinity tool for Immunoglobulin Isolation and immunological experiment
A hybrid protein comprising ATF domain of pro-UK and VAS, an angiogenesis inhibitor, is a potent can
利用荧光标记引物和DNA自动测序仪确定DNA的断裂位点
Expression and Purification of Intact and Functional Soybean (Glycine max) Seed Ferritin Complex in
Fusion protein between protein ZZ and red fluorescent protein DsRed and its application to immunoass
利用酵母双杂交系统检测微囊藻毒素
P6BMP2的克隆及其在大肠杆菌中的表达
壳聚糖纳米颗粒的制备及其质粒转染研究
Impairment of Na+,K+-ATPase in CD95(APO-1)-induced human T-cell leukemia cell apoptosis mediated by
Polyethylenimine-complexed plasmid particles targeting Focal Adhesion Kinase Function as Melanoma Tu
A novel ferritin gene, SferH-5, reveals heterogeneity of the 26.5-kDa subunit of soybean (Glycine ma
Cross-linked Polyethylenimine as Potential DNA Vector for Gene Delivery with High Efficiency and Low
AUF1-like protein binds specifically to DAS cis-acting element that regulates mouse alpha-fetoprotei
人血管内皮抑制素vasostatin120-180在大肠杆菌中的表达纯化及初步的抗血管生成活性研究
Combination of human plasminogen kringle 5 with ionizing radiation significantly enhances the effica
Fusion Protein between Protein ZZ and Enhanced Green Fluorescent Protein and its Application to Immu
Sinomenine inhibits primary CD4+T cells proliferation via apoptosis
雷公藤内酯醇人工抗原的合成及多克隆抗体的制备
鸡局部黏着斑激酶基因5′UTR及启动子的克隆鉴定
Comparison of gene expression profiles in mouse primary T cells under normal and prolonged activatio
肌钙蛋白Ⅰ的可溶性表达及纯化研究
TDG蛋白的表达纯化及多克隆抗体的制备
基于酵母双杂交的微囊藻毒素检测系统初探
Cross-linked Polyethylenimine as Potential DNA Vector for Gene Delivery with High Efficiency and Low Cytotoxicity
Expression of Recombinant Human FADD, Preparation of Its Polyclonal Antiserum and the Application in Immunoassays
三种脂类物质对T细胞的影响
pT7MT, a Metallothionein 2A-ta
Tumor-targeting Salmonella typ
Application to immunoassays of
Impairment of Na+, K+-ATPase i
AUF1-like protein binds specif
壳聚糖纳米颗粒的制备及其质粒转
Immobilized protein ZZ, an Aff
Expression, Purification and Characterization of C-FADD
利用荧光标记引物和DNA自动测序仪确定DNA的断裂位点
壳聚糖纳米颗粒的制备及其质粒转染研究
鸡局部黏着斑激酶基因5′UTR及启动子的克隆鉴定
FADD and its Phosphorylation
Combination of human plasminog
Production of the Polyclonal A
A Novel Genotyping Strategy Ba
利用荧光标记引物和DNA自动测序
Efficient Gene Transfection in
An improved strategy for high-
谷胱甘肽亲和色谱介质的制备及对
Fusion Protein between Protein
Cross-linked Polyethylenimine
TDG蛋白的表达纯化及多克隆抗体的制备
Cross-linked Polyethylenimine as Potential DNA Vector for Gene Delivery with High Efficiency and Low Cytotoxicity
Expression of Recombinant Human FADD, Preparation of Its Polyclonal Antiserum and the Application in Immunoassays
三种脂类物质对T细胞的影响
期刊信息
  • 《生物化学与生物物理学报：英文版》
  • 北大核心期刊（2004版）
  • 主管单位:
  • 主办单位:中国科学院上海生物化学研究所
  • 主编：
  • 地址：上海岳阳路319号
  • 邮编：200031
  • 邮箱：abbs@sibs.ac.cn
  • 电话：021-54920956 54920955
  • 国际标准刊号：ISSN：1672-9145
  • 国内统一刊号：ISSN：31-1940/Q
  • 邮发代号:4-210
  • 获奖情况:
  • 国内外数据库收录:
  • 美国化学文摘（网络版）,英国农业与生物科学研究中心文摘,荷兰文摘与引文数据库,美国生物医学检索系统,美国剑桥科学文摘,美国科学引文索引（扩展库）,美国生物科学数据库,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊（2004版）,英国英国皇家化学学会文摘,中国北大核心期刊（2000版）
  • 被引量:5851