中文摘要：

将人工合成的来自Escherichia coli的dsdA基因与来自Schizosaccharomyces pombe的dao1基因,通过农杆菌侵染方式转化到水稻中花11中。对转化植株进行分子生物学检测表明,目的基因整合到了水稻核基因组中,其中获得转化dsdA的单拷贝植株7个,转化dao1的单拷贝植株6个。通过对单拷贝转基因家系表达量检测及T0代种子萌发测验发现,dao1基因在2个（编号6,7）转基因家系中表达量显著,T0代种子在含D-Ala的培养基上,具有明显抗性。dsdA基因在所有的转基因家系中表达量相对偏低,在含D-Ser的培养基上,T0代种子有一定抗性。

英文摘要：

Two new selectable marker gene dsdA and daol from Escherichia coli and Schizosac- charomyces were synthesized by codon-optimization, and then transformed into japonica rice cultivar zhonghua 11 through Agrobacterium mediated gene transformation. Southern hybridization of the DNA transformants showed that 7 single-copy plants ofdsdA gene and 6 single-copy plants of daol gene were obtained. Results of detecting expression levels of the single-copy transformants and the germination texts of To generation seeds showed that there were two single-copy gene transformants （numbered as 6 and 7） of daol gene,indicating high expression levels and consistent with seed germination testing on D-Ala. In all single-copy transformants of dsdA gene,the expression levels of dsdA were relatively low. Test of seed germination showed that there was the resistance to D-Ser. This study initially explored the application of dsdA,daol as new selectable marker genes for rice nuclear transformation, and will lay the foundation for its extensive applications.