中文摘要：

目的：研究车前子含药血清对大鼠颅骨成骨细胞体系增殖、分化及成骨能力的影响,并初步探讨上皮钠离子通道（ENaC）参与车前子调节成骨细胞功能的作用。方法：按血清药理学实验方法制备车前子含药血清和对照血清,采用酶消化法分离培养大鼠成骨细胞,CCK-8试剂盒和AKP试剂盒分别测定车前子含药血清对细胞增殖和分化的影响,半定量PCR方法测定车前子含药血清对成骨细胞ENaC和成骨功能指标基因表达的影响。结果：不同浓度的车前子含药血清均能显著刺激成骨细胞增殖、分化,其促进作用与药物浓度呈正相关关系,具有浓度依赖性;与对照组比较,低浓度车前子含药血清能显著上调成骨细胞α-ENaC基因的表达水平,其增加趋势与成骨功能指标基因（OC、ALP、OP）mRNA表达的增加趋势一致。结论：车前子含药血清不仅刺激成骨细胞增殖分化,在促进骨形成的同时也增加α-ENaC mRNA表达,提示其作用机理可能与ENaC介导的调节途径有关,为中药治疗骨代谢疾病的研究提供了一个新思路。

英文摘要：

Objective：To study the influence of Plantaginis Semen on cell proliferation,differentiation and function of rat osteoblasts, and investigate the regulation effects of rat osteoblast epithelial sodium channel（ENaC） on bone formation. Methods ：The animal serum was prepared by serum pharmacology means. The cells were got by separating and inducing the SD neonatal rat＇s skull bone. Cell proliferation and differentiation were evaluated by CCK-8 assay kit and AKP assay kit respectively. Regulation effects on mRNA expression of ENaC and osteogenesis gene were investigated by semi-quantitative PCR. Resuhs：Plantaginis Semen stimulated the osteoblasts proliferation and differentiation, the difference between treatment group and control group had statistical significance（P 〈 0. 01 ）in a dose-dependent man- ner. The effects of Plantaginis Semen serum on a-ENaC gene expression paralleled those on osteogenic gene （OC, ALP, OP） expression level. Conclusion：Plantaginis Semen stimulates proliferation, differentiation and the mRNA expression of ENaC and osteogenesis gene in rat osteoblasts. Our results suggest that ENaC participate in the effects of Plantaginis Semen serum on osteoblast bone formation. Regulation of ENaC channel expression and function may provide a new clue for research on treatment of osteoporosis with traditional Chinese medicine.