中文摘要：

目的：评价激光多普勒血流仪（LDF）在大鼠脑缺血再灌注模型制作中的应用效果。方法：Wistar大鼠分为对照组和实验组。线栓法建立大脑中动脉阻塞（MCAO）模型。实验组在LDF监测下制作模型，当栓线插入深度约为18～20rain时，有轻微阻力，同时LDF显示血流下降至基础值的30％以下，即为栓塞成功，于1h后拔栓线见血流明显回升，即为再灌注成功；对照组按传统法当栓线插入深度约为18~20mill时，有微阻力即认为栓塞成功，于缺血1h后拔栓线实现再灌注。12h后行神经功能评分；48h后2，3，5-氯化三苯基四氮唑（TTC）测定脑梗死。结果：对照组认为插线成功的大鼠仍有14．3％无神经损伤症状，出现缺损症状的大鼠约有13．3％未出现梗死灶。实验组经LDF判断模型成功的大鼠均出现神经损伤症状和较稳定的梗死灶。结论：应用LDF能显著提高MCAO模型复制的成功率和模型的均衡性。

英文摘要：

Objective： To evaluate the effect of laser doppler flowmeter （LDF） on the establishment of rat focal cerebral ischemia/reperfusion model. Methods： Wistar rats （280 - 320 g） were randomly divided into 2 groups： a control group and an experimental group. The ischemia model of both groups was established by left middle cerebral artery occlusion （MCAO）. The experimental model was duplicated under the LDF monitoring： there was a slight resistance when the occlusion line was inserted as deep as about 18 mm to 20 mm, meanwhile LDF showed blood flow fell to below 30% of the based value, which is defined as successful embolism. The blood flow rebounded significantly after reperfusion by gently pull out the occlusion line 1 h after ischemia, which meant a successful reperfusion The cerebral blood flow of the control group was not monitored in the process of model establishment. Neurobehavioral score was evaluated according to Zea Longa et al. at 12 h after reperfusion and cerebral infarction volume was identified by TTC staining at 48 h after reperfusion. Results： No obvious neurologic injury symptoms appeared in 14. 30% rats of the control group. No obvious infarct appeared in 10% rats after TTC staining in which there were neural defective symptoms. The successful MCAO model rats judged by LDF in the experimental group showed nerve function injury symptoms and TTC staining showed more stable regional infarcts. Conclusion： Early auxiliary judgment basis of the MCAO model can significantly increase the success rate of model replication and the model equilibrium to monitor blood flow changes simultaneously during model establishment.