中文摘要：

利用新型3′，6′-对戊酰硼荧光素探针，结合超氧化物歧化酶（S0D）的选择性催化歧化反应特性，建立了一种超氧阴离子（02^-）的酶催化荧光分析方法。SOD催化黄嘌呤／黄嘌呤氧化酶体系中的02^-生成H2O2，使得荧光探针转换为强荧光的荧光素产物，从而实现对02^-的荧光检测。该方法检测线性范围为2．4×10^-7～1．5×10^-5sm01／L，检测下限1．0×10^-7mol／L，显示了较高选择性和灵敏度。

英文摘要：

A enzymatic assay for superoxide anion （02^- ） has been developed by using a new H2O2 fluorescent probe, 3＇, 6＇ - bis（pinacolatoboron） fluoran, as the fluorescent substrate of superoxide dismutase （SOD） . The superoxide anion in xanthine/xanthine oxidase system was catalyzed by SOD to form H2O2, resulting in a clear increase in the fluorescence intensity of the fluorescent probe. The experimental results show that there is a linear relationship between the fluorescence intensity and the concentration of superoxide anion in the range of 2.4 x 10^-7 1.5 x 10^-5 mol/L and the detection limit reaches 1.0 x 10^-7 mol/L.