以高抗旱油用向日葵自交系K 58与低抗旱油用向日葵自交系K 55为亲本,由其组配的F1代杂交种和通过单粒传方法获得的187个F5RILs群体为试验材料,设8组聚乙二醇(PEG-6000)浓度梯度,用亲本及杂交种F1代进行条件摸索试验,筛选出干旱临界浓度为20%PEG-6000,用此浓度对其余187个重组自交系材料进行干旱胁迫试验,研究干旱胁迫对向日葵种子萌发的影响,为向日葵抗旱性研究及后期QTL定位提供科学理论依据。结果表明,187份向日葵种子的发芽率、发芽势、发芽指数、胚根长、胚芽长、胚根干鲜重及胚芽干鲜重在干旱胁迫下较正常供水条件呈现不同程度的下降;在正常供水及模拟干旱2种条件下,各项性状指标在187株系间均呈现出显著或极显著差异,且呈正态分布,指标间显现为显著或极显著的正相关性。
In this study,with two optional inbred lines K 55,K 58 as parents,it crossed F1 generation,F1 selfing got F2,finally obtained a F5 RILs populations which contain 187 plants by single-seed descent method as the experimental material.It had 8groups of polyethylene glycol(PEG-6000)concentration gradient for parents and hybrid F1 generation conditions for experiment,screened drought critical concentration of 20% PEG-6000 was screened to carnied the droaght stress test with the remaining 187 recombinant inbred lines material.The aim of this study is to study the effects of drought stress on sunflower seed germination,to provide a theoretical basis in terms of research on drought resistance of sunflower later and on QTL mapping.The results showed that the germination rate,sprout potential,germination index,embryonic bud lenth,embryonic root length,embryonic bud and embryonic root fresh weight and dry weight decreased.With the increasing of drought concentration,the inhibition enhanced gradually in F5 RILs.Every character showed significant or over significant differences in experimental materials under two different conditions,normal water and drought.Besides,it presents normal distribution and shows significant or over significant positive correlation between characters.