应用AFLP技术对赤点石斑鱼Epinephelus akaara7个地理群体进行了遗传多样性及遗传分化的分析。结果显示:不同群体的遗传多样性差异较大,大亚湾群体和舟山群体遗传多样性最低,湛江群体最高;通过UPGMA聚类,7个群体88个个体明显分成3支,三亚群体单独聚为一支(Clade A),湛江群体的部分个体聚类为一支(Clade B),湛江群体剩余个体和其他5个群体的个体聚为一支(Clade C)。其中在分支C中存6个小的分支,这6支中个体间基本以地理群体进行聚类。研究结果为赤点石斑鱼种质资源保护和遗传改良提供了遗传学依据。
The genetic diversity and genetic differentiation of Epinephelus akaara populations in seven geographical regions were analyzed by Aamplified Fragment Length Polymorphism (AFLP) technique. The analysis results revealed that the levels of genetic diversity among the 7 populations were different. It has been shown that the genetic diversity of E. akaara populations in Dayawan (DYW) and Zhoushan (ZS) were the lowest, and the genetic diversity of E. akaara population was the highest. Eighty-eight samples in 7 E. akaara populations could be apparently classified into three clades by UPGMA analysis, E. akaara population in Sanya (SY) was categorized into clade A. part of E. akaara population in Zhanjiang (ZJ) was categorized into the clade B. and other part of E. akaara population in Zhanjiang (ZJ), as well as rest of five E. akaara population were categorized into clade C. The six subclades were further clarified among clade C according to the geographical distribution. Taken together, our study would provide valuable information for the genetic conservation and improvement of E. akaara.