中文摘要：

为探讨X射线联合沙利度胺对胶质瘤U251细胞的抑制作用，采用H3-TdR掺入法和细胞克隆形成法检测沙利度胺增强U251细胞的辐射敏感性；细胞划痕实验观察沙利度胺抑制U251细胞的浸润能力。结果表明，沙利度胺能够抑制U251细胞浸润、DNA合成和克隆形成，与X射线联用后抑制作用增强，表现出协同作用。当细胞存活率为50％时，60μg／mL、100μg／mL沙利度胺的辐射增敏系数为1．18和1．51。实验证明X射线联合沙利度胺能显著提高胶质瘤U251细胞的辐射敏感性，对U251细胞具有辐射增敏作用。

英文摘要：

In order to investigate the inhibition effect of X-rays combined with thalidomide on glioma U251 cells, the cell scratch assay was used to detect the inhibition of thalidomide on U251 cells. And H3-TdR incorporation assay and colony formation were used to investigate the enhancement effect of thalidomide on the sensitivity of U251 cells to X-rays. It has been found that thalidomide has synergistic effect on therapy of glioma U251 cells if it is com- bined with X-rays irradiation as it can inhibit cells infiltration, DNA synthesis and colony formation. When the survival rate of glioma U251 cells is 50%, the radio-sensitization factors of 60 μg/mL and 100 μg/mL thalidomide is 1.18 and 1.51 respectively. The results reveal that thalidomide could significantly improve radio-sensitivity of glioma U251 cells.