中文摘要：

目的：建立高灵敏度测定蜂蜜中四环素总量的电化学发光法。方法：基于四环素、土霉素、金霉素、强力霉素对钌联吡啶的电化学发光具有几乎完全相同的增强效应,建立本方法,并阐明其机理。结果：在最优实验条件下,钌联吡啶的电化学发光增强值与四环素、土霉素、金霉素、强力霉素的浓度的对数呈线性关系,线性范围为1.0×10-12～5.0×10-10 mol.L-1,检测限约为3×10-13 mol.L-1。精密度和准确度好。机理研究表明：四环素的电化学氧化产物增强了钌联吡啶的电化学发光。结论：该方法灵敏度极高,测定结果准确可靠,可用于蜂蜜中四环素总量的测定。

英文摘要：

Objective：To establish an electrochemiluminescence（ECL） method with high sensitivity to determine the total amount of tetracyclines in honey.Method：The ECL of Ru（bpy）32＋ could be enhanced by tetracycline（TC）,oxytetracycline（OTC）,chlortetracycline（CTC） and doxycycline（DC） identically.Based on it,this method was developed.And the ECL enhancement mechanism was studied.Results：Under the optimized conditions,the enhanced ECL intensity versus the logarithm of the concentration of TCs is in good linear relationship over a concentration range from 1.0×10-12 to 5.0×10-10 mol·L-1,with the detection limit 3×10-13 mol·L-1.The precision and accuracy are good.The mechanism studies showed that the oxidation production of TCs could enhance the ECL of Ru（bpy）32＋.Conclusion：The proposed method is highly sensitive,accurate and reliable,and thus then can be used for the ultrasensitive determination of the total amount of tetracyclines in honey.