从生活垃圾堆积地采集土样,通过平板初筛和液体摇瓶复筛得到1株高效兼溶5种难溶磷的溶磷细菌菌株SY0,培养6 d后该菌株对卵磷脂、磷酸钙、磷酸铁、磷酸铝及磷矿粉的溶磷量分别可达23.1、598.1、14.3、18.6、43.2 mg/L,经过生理生化及16S r DNA序列鉴定,初步确定SY0为巨大芽孢杆菌(Bacillus megaterium)。利用滤纸法和盆栽试验研究该菌株对花生生长发育的影响发现,该菌株菌液100倍稀释液处理的种子发芽势比对照(无菌水处理)提高30.23%。施入SY0菌液后花生出苗时间提前2 d;苗期地上部分鲜质量、干质量、叶绿素含量、根系鲜质量、根系干质量、根系活力分别比对照(自来水处理)显著增加33.24%、19.80%、28.59%、51.25%、38.89%、51.83%;开花时间提前2 d,开花相对集中;生物产量、百仁质量和荚果产量分别比对照显著提高7.97%、8.29%和9.88%。
A bacterium strain capable of dissolving five kinds of insoluble phosphates was isolated from the living garbage dumping soil by plate screening and liquid submerged culture,named as SY0. By use of the bacterium strain SY0,the water-soluble phosphorus concentration could be increased to 23. 1,598. 1,14. 3,18. 6 and 43. 2 mg / L after six days treated by lecithin,tricalcium phosphate,ferric phosphate,aluminium phosphate and ground phosphate rock respectively. The strain SY0 was identified as Bacillus megaterium by physiological identification and 16 S r DNA gene sequencing analysis. The effects of the strain on peanuts growth were studied by the filter paper method and pot experiment. The results showed that after treated with 100-fold dilution of the original bacterial culture,the germination potentiality of peanut increased by 30. 23%. After treated by SY0,the peanut seeds sprouted two days earlier,the chlorophyll content increased by 28. 59%,the root activity increased by 51. 83%,and the weight of fresh above-ground plant,dry above-ground plant,fresh root system and dry root system increased by 33. 24%,19. 80%,51. 25% and 38. 89% respectively. Moreover,after treated by the bacterium strain SY0,the flowers appeared two days earlier,the flowering stage was centered,and the biological yield,hundred kernel weight and pod yield increased by 7. 97%,8. 29% and 9. 88% respectively.