中文摘要：

氯过氧化物酶（ CPO）催化苯酚与H20 2发生过氧化反应生成邻苯二 酚 , 能减轻苯酚对 降解菌株的抑制作用,加快降解菌株对苯酚的生物降解.实验结果表明：在2 h 内适量的H2O 2 存在时10 U/L的 CPO可以使300 mg/L苯酚降解率达到6 7 . 8 5 %,而 CPO与降解菌株协同作 用下苯酚降解率则可达到70. 72%,比单-菌株降解率8.52%提高了 62. 2%.在降解体系中补 充邻苯二驗进一步揭示了 CP0氧化苯酚的中间产物有利于菌体细胞形成共基质效应,提高细 胞的苯酚生物降解效率.降解动力学分析显示：在苯酚质量浓度为100 -1 200 mg/L 时, CP0 与菌株协同降解体系的最大比降解速率gmax =0.000195 h -1,基质饱和常数=1.0501 mg/L, 基质抑制常数K1 =5.1272 mg/L.

英文摘要：

Chloroperoxidase （CPO） can catalyze phenol reacting peroxide reaction generating with H2O2 to catechol,which can re-duce the inhibitory effect of phenol degradating generating bacterial strains. At the meantime,it can accelerate the rate of phenols biodegradation. The results show that 10 U/L of CPO has 67. 85% conversion rate of 300 mg /L phenol within 2 h with an appropri-ate amount of H2O2. While the degradation rate of phenol degradation under synergy of strain and CPO is up to 70. 72% increased by 62.2% comparing with a single strain degradation rate （8.52% ） . Supplementary catechol in the reaction system can further ver-ified that the intermediate products can be good for forming the co-substrate effect in bacteria, thereby improving the phenol degrada-tion efficiency of biological bacterial cells. Biodegradation dynamics analysis shows that the maximum specific degradation rate of the CPO and strain synergistic. The maximum specific degradation rate qmax = 0. 000195 h 1 , the matrix saturation constant Ks = 1.0501 mg/L,and the substrate inhibition constant Kj = 5. 1272 mg/L when phenol concentration in the range of 100 - 1 200 mg/L.