中文摘要：

目的探讨Pydn重组蛋白对支气管哮喘小鼠气道炎症的抑制作用及机制的研究。方法40只雄性清洁级BALB／c小鼠，随机分为4组，每组10只。分别为生理盐水对照组、卵蛋白（OVA）哮喘组、Pyrin重组蛋白治疗3d组和Pyrin重组蛋白治疗7d组。在末次激发24h后所有小鼠取左肺组织行苏木精．伊红（HE）染色，Masson三色染色；用图像分析软件测定支气管壁厚度（WAt／Pbm）、支气管平滑肌厚度（WAm／Pbm）及胶原纤维面积；用酶联免疫吸附法（ELISA）检测支气管肺泡灌洗液（bronchoalveolarlavage：fluid，BALF）中IL-4、IL-5、TNF—α及IFN-1含量；用RT—PCR检测肺组织中结缔组织生长因子（connectivetissuegrowthfactor，CTGF）和转化生长因子-β1（transforminggrowthfactor-61，TGF—β1）的mRNA表达；用Westernblot方法检测核转录因子（nuclearfactorkappaB，NF-KB）的表达。结果哮喘模型组小鼠BALF中细胞总数、IL4、IL-5、TNF-仪水平增高和IFN-1水平降低；肺组织支气管管壁厚度、气道平滑肌厚度、胶原纤维面积，CTGF、TGF-β1的转录和表达水平以及NF—κB表达水平均显著高于生理盐水对照组（P〈O．05）。Pyfin重组蛋白3d和7d干预组小鼠BALF中细胞总数、IL-4、IL-5、TNF-α水平和肺组织支气管管壁厚度、气道平滑肌厚度、胶原纤维面积、NF—κB表达水平显著降低，与哮喘模型组比较差异均具有统计学意义（P〈0．05），而BALF中IFN-γ水平明显上升，与哮喘模型组比较，差异均具有统计学意义（P〈O．05）；P州n重组蛋白7d干预组CTGF、TGF-β1的转录和表达水平显著低于哮喘组（P〈0．05）。结论我们推测Pyrin重组蛋白可能部分是通过抑制NF-κB信号途径来阻断TGF-β1和CTGF的过度表达而实现抑制气道炎症，进而抑制气道重构的发生。

英文摘要：

Objective To investigate the blocking effects of Pyrin domain protein on mouse aller- gic airway inflammation induced by ovalbumin and its mechanism. Methods Forty male BALB/c mice were randomly divided into 4 groups with 10 mice in each group. Control group： mice were treated with saline; OVA group ： mice were sensitized and challenged With OVA; Pyrin domain protein 3 d group ： mice were sen- sitized and challenged as asthmatic group and treated with 100 μg/kg triptolide before challenged; Pyrin do- main protein 7 d group ： mice were sensitized and challenged as asthmatic group and treated with 100 μg/kg triptolide before challenged. All mice were killed 24 h after final OVA challenge. The left lung was isolated for pathological examination. Lung sections were stained with hematoxylin and eosin（HE）, Masson＇s tri- chrome. The thickness of bronchial airway, bronchial smooth muscle thickness, and the collagen deposition area were measured by image analysis system. The concentrations of IL-4, IL-5, TNF-α and IFN-γin BALF were measured by ELISA, the RT-PCR and Western blot was performed to detect the mRNA expression of connective tissue growth factor （CTGF）, TGF- β 1 mRNA and NF - κB from the right lung tissues. Results In OVA group, the number of inflammatory cells and the.concentrations of IL-4, IL-5, TNF-α in BALF andBronchial airway thickness, bronchial smooth muscle thickness, the collagen deposition area, CTGF, TGF- β1 mRNA and NF-KB in lung tissue were significantly higher than those in control group（P〈0.05 ）. IFN-γ, were lower than those in control group（ P〈0.05 ）. While in Pyrin domain protein 3 d group and Pyrin domain protein 7 d group, the number of inflammatory cells, the concentrations of IL-4, IL-5, TNF-α in BALF and bronchial airway thickness, bronchial smooth muscle thickness, the collagen deposition area and NF-KB in lung tissue were significantly lower than those in OVA group, IFN-3, were higher than those in OVA group（ P 〈0.05）. In Pyrin domain protein