中文摘要：

为了控制一类传染病-鲤鱼春季病毒血症（SVC）,本文亚克隆了SVC中国株糖蛋白基因（SVCV-C1 G）,并采用pET21a-SVCV-C1 G/BL21（DE3）表达系进行了表达。随后,纯化了SVCV-C1 G蛋白,纯化蛋白分子量为49.5 kDa。SVCV-C1 G蛋白由442个氨基酸组成,与其他弹状病毒糖蛋白的同源性在18.4%~99.0%。系统进化树显示,SVCV-C1 G属于Ia亚型。研究结果为进一步研制SVC基因工程疫苗奠定了基础。

英文摘要：

In order to control spring viremia of carp（SVC）,the glycoprotein（G） of spring viremia of carp virus Chinese isolation（SVCV-C1 G） was subcloned and expressed in the pET21a/BL21（DE3） system.SVCV-C1 G protein was purified and analyzed using SDS-PAGE.SVCV-C1 G is comprised of 442 amino acid,and the molecular weight is 49.5 kDa.In comparison of other rhabdovirus G proteins with SVCV-C1 G,the identity is 18.4%~99.0%.Phylogenetic analysis shows that SVCV-C1 G belongs to the Ia subtype.The results are useful for the development of molecular vaccine of SVC.