中文摘要：

在 Bombyx mori nucleopolyhedrovirus (BmNPV ) 的迟了的阶段感染，大量 polyhedra 出现在感染的房间核，是稠密的这些 polyhedra 保护合并 virions 免受严厉环境的伤害的蛋白质晶体。调查外国蛋白质是否能被使不能调动进 BmNPV 的 polyhedra ，二 recombinant baculoviruses 被新奇 BmNPV polyhedrin加(polh+)产生 Bac-to-Bac 系统，当 vBmBac (polh+)提高了绿色，指明了荧光灯蛋白质( EGFP )和 vBmBac (polh+)能同时表示 polyhedrin 和外国蛋白质的-LacZ,。轻显微镜学分析证明所有病毒生产了正常外观的 polyhedra。格林荧光能显然在 vBmBac (polh+) 的表面上被检测 -EGFP polyhedra，然而并非 BmNPV polyhedra。荧光分析并且反干燥测试证实 EGFP 在 polyhedra 被嵌入。是期望， vBmBac (polh+)-LacZ polyhedra 包含了 LacZ 的数量并且举办了一项更高的牛乳糖活动。如果外国蛋白质被使不能调动进 polyhedra，钠 dodecyl 硫酸盐 polyacrylamide 胶化电气泳动(SDS 页) 并且西方的弄污也被执行验证。这研究为有用蛋白质的有效保藏和新杀虫剂的开发向新灵感提供有毒的蛋白质。

英文摘要：

in the late phase of Bombyx mori nucleopolyhedrovirus （BmNPV） infection, a large amount of polyhedra appear in the infected cell nucleolus, these polyhedra being dense protein crystals protecting the incorporated virions from the harsh environment. To investigate whether the foreign protein could be immobilized into the polyhedra of BmNPV, two recombinant baculoviruses were generated by a novel BmNPV polyhedrin-plus （polh＋） Bac-to- Bac system, designated as vBmBac（polh＋）-enhanced green fluorescent protein （EGFP） and vBmBac（polh＋）-LacZ, which can express the polyhedrin and foreign protein simultaneously. Light microscopy analysis showed that all viruses produced polyhedra of normal appearance. Green fluorescence can be apparently detected on the surface of the vBmBac（polh＋）-EGFP polyhedra, but not the BmNPV polyhedra. Fluorescence analysis and anti-desiccation testing confirmed that EGFP was embedded in the polyhedra. As expected, the vBmBac（polh＋）-LacZ polyhedra contained an amount of LacZ and had a higher β-galactosidase activity. Sodium dodecyl sulfate polyacrylamide gel electrophoresis （SDS-PAGE） and Western blotting were also performed to verify if the foreign proteins were immobilized into polyhedra. This study provides a new inspiration for efficient preservation of useful proteins and development of new pesticides with toxic proteins.