中文摘要：

目的：利用报告基因监测肝干细胞向胰腺细胞的转分化潜能。方法：通过PCR从大鼠鼠尾基因组中克隆胰岛素启动子片段,构建大鼠胰岛素Ⅰ启动子调控的红色荧光蛋白报告载体（pRIP Ds Red）,并利用报告载体标记的肝原始细胞系（liver epithelialprogenitor cells,LEPCs）及经逆转录病毒感染后表达MafA的肝原始细胞系（LEPCs-MafA）,观察肝干细胞向胰腺细胞的转分化潜能。结果：获得胰岛素启动子调控的报告载体、报告载体标记的LEPCs及LEPCs-MafA。结论：pRIP DsRed报告载体的成功构建为研究LEPCs向胰腺细胞的转分化提供了简捷的分子工具。

英文摘要：

OBJECTIVE：To construct the report gene expression vector for studying the transdifferentiation potency of liver epithelial progenitor cells（LEPCs）.METHODS：The rat insulin Ⅰ promoter（RIP）was amplified by PCR from rat tail genomic DNA and subcloned into promoter-removed pDsRed1-1 vector.By using the reporter gene tagging cells,the phenotype variation of LEPCs transdifferentiate into pancreas-like cells could be evaluated.RESULTS：RIP report gene expression vector could monitor the transdifferentiation of LEPCs into pancreas-like cells in a real-time manner.CONCLUSION：The reporter vector could useful for studying the transdifferentiation of LEPCs into pancreas-like cells.