中文摘要：

本文采用15％KOH的醇溶液除去小鼠单核巨噬细胞RAW264．7样品中的甘油三酯，用6％三氯乙酸除去其中蛋白质，然后以体积比为3：2的正己烷与异丙醇混合溶剂提取巨噬细胞内的胆固醇。采用硬脂酸键合硅胶色谱柱，以乙腈和异丙醇的混合溶剂为流动相，在检测波长212nm下测定胆固醇的含量。结果表明，胆固醇含量在0．005～0．3g／L之间时具有较好的线性关系，相对标准偏差为3．4％，检出限为1．5mg／L，回收率为96．04％。方法用于测定巨噬细胞中胆固醇的含量，结果满意。

英文摘要：

An equal volume of freshly prepared KOH in ethanol （150 g/L） was added to monocyte-derived macrophages to hydrolyze the triglyceride, and 6% trichloroacetic acid was added to remove the protein. And then, the cholesterol of macrophages was extracted with hexane-isopropanol （3： 2, V/V）. The cholesterol was analyzed using high performance liquid chromatography （HPLC）. The C18 alkylamide silica column （150×4.6 mm, 5 μm） was selected and the eluent consisted of isopropanolacetonitrile 30：70（V/V） at a flow rate of 1. 0 mL/min. The detection wavelength was 212 nm. The calibration curve is linear in the range of 0.005-0.3 g/L for cholesterol （r=0. 9996）. The detection limit is 1.5 mg/ L, relative standard deviation （RSD） is 3.4%, and the value of recovery is 96.04%. The method is rapid, accurate and reliable.