中文摘要：

瞄准：与肝炎 B (HBV ) 复制建立一个快速、方便的动物模型。方法：HBV-replication-competent 原生质标志的一个裸体 DNA 解决方案经由尾巴静脉被转移到 BALB/C 鼠标，用一个水动力学在活体内 transfection 过程。在注射以后，这些老鼠在 d 上被牺牲 1， 3， 4， 5， 7 和 10。在肝的 HBV DNA 复制中介被南部的污点杂交分析。肝炎 B 核心抗原(HBcAg ) 和在肝的肝炎 B 表面抗原(HBsAg ) 的表示被免疫组织化学检查。浆液 HBsAg 和肝炎 B e 抗原(HBeAg ) 被连接酶的免疫吸着剂试金(ELISA ) 检测。HBV 复制的抑制在与 polyinosinic-polytidylin 酸(polyIC ) intraperitoneally 对待的 HBV 复制模型老鼠被比较或缓冲磷酸盐 saline (PBS ) 。结果：在水动力学在活体内 transfection 以后，在老鼠肝的 HBV DNA 复制中介在 d 上是可检测的 1 并且在 d 上丰富 3 和 4，层次稍微被减少并且在 d 之间仍然保持相对稳定 5 和 7，并且在 d 上是几乎无法发现的 10。HBcAg 和 HBsAg 的表示模式类似于 HBV 复制中介 DNA 的，除了他们在 d 上到达了一座山峰之外 1 在注射以后。在 HBV DNA 复制中介的明显的差别都没在肝的左、正确、中间的脑叶被观察。有 polyIC 的术后疗法，在肝的 HBV 中间的 DNA 的水平在与 PBS 注射的控制老鼠是比那低的。结论：有 HBV 复制的高水平的一个快速、方便的老鼠模型被开发并且过去常在 HBV 复制上调查 polyIC 的禁止的效果，它为未来提供一个有用工具 HBV 染色体的功能的研究。

英文摘要：

AIM： To establish a rapid and convenient animal model with hepatitis B virus （HBV） replication. METHODS： A naked DNA solution of HBV-replicationcompetent plasmid was transferred to BALB/C mice via the tail vein, using a hydrodynamic in vivo transfection procedure. After injection, these mice were sacrificed on d 1, 3, 4, 5, 7 and 10. HBV DNA replication intermediates in the liver were analyzed by Southern blot hybridization. The expression of hepatitis B core antigen （HBcAg） and hepatitis B surface antigen （HBsAg） in the liver was checked by immunohistochemistry. Serum HBsAg and hepatitis B e antigen （HBeAg） was detected by enzyme- linked immunosorbent assay （ELISA）. Inhibition of HBV replication was compared in HBV replication model mice treated intraperitoneally with polyinosinic-polytidylin acid （polyIC） or phosphate-buffered saline （PBS）. RESULTS： After hydrodynamic in vivo transfection, HBV DNA replication intermediates in the mouse liver were detectable on d 1 and abundant on d 3 and 4, the levels were slightly decreased and remained relatively stable between d 5 and 7, and were almost undetectable on d 10. The expression patterns of HBcAg and HBsAg were similar to that of HBV replication intermediate DNA, except that they reached a peak on d 1 after injection. No obvious differences in HBV DNA replication intermediates were observed in the left, right and middle lobes of the liver. After treatment with polyIC, the level of HBV intermediate DNA in the liver was lower than that in the control mice injected with PBS. CONCLUSION： A rapid and convenient mouse model with a high level of HBV replication was developed and used to investigate the inhibitory effect of polyIC on HBV replication, which provides a useful tool for future functional studies of the HBV genome.