中文摘要：

【目的】建立龙血竭中新化合物一剑叶龙血素C的分离与测定含量方法。【方法】采用Phenomenex C18（250mm×4.6mm，5μm）色谱柱，甲醇-水（75：25，v／v）为流动相，流速为1m／min，检测波长为295nm，柱温50℃；用外标法定量。【结果】剑叶龙血素C在27．25—327ng范围有良好线性关系，r=0．9997，理论塔板数以剑叶龙血素C计为4500；方法回收率为96．90％，RSD为1．2％。【结论】本方法是测定龙血竭中剑叶龙血素含量的准确的定量方法。

英文摘要：

[Objective] To establish a HPLC method for the separation and determination of Cochinchinenin C in Dragon＇ s Blood. [Methods] The chromatographic system consisted of Phenomenex C18 （250 mm×4.6mm,5μm） column and mobile phase of CH3OH-H2O （75 ： 25） and detected at 295 nm, the temperature of column was 50℃ and quantitating with external standard method. [Results] The standard curves of Cochinchinenin C was linear in the concentration range of 27.25 - 327 ng （ r = 0.9997）. The theoretical plate number is 4500. The average recovery rate was 96.90% （RSD =1.2%）. [Conclusions] The established method is accurate, sensitive, simple and reliable for determination of Cochinchinenin C in Dragon＇s Blood.