中文摘要：

为了从油菜中克隆BnM KS1基因并且了解其诱导表达情况,以甘蓝型油菜品种中双9号为材料,采用同源克隆法克隆获得甘蓝型油菜MKS1（BnMKS1）的cDNA序列.BnMKS1编码的蛋白质含有217个氨基酸残基,与AtMKS1有82％的相似性.序列分析结果表明BnMKS1含有1个VQ结构域和8个潜在的MAP kinase磷酸化位点.农杆菌介导的烟草瞬时表达分析结果显示,BnMKS1是一个细胞核定位蛋白质.定量RT-PCR分析结果显示,菌核病菌（核盘菌）、甲基茉莉酸以及苯丙噻重氮强烈诱导BnMKS1的表达,而脱落酸对其表达没有影响.说明BnMKS1在油菜对菌核病菌Sclerotinia.sclerotiorum的防卫反应中起作用.这些结果将为进一步深入研究BnMKS1在油菜与核盘菌相互作用中的功能提供有用的信息.

英文摘要：

To clone BnMKS1 gene and define its response to pathogenic cues in oilseed rape, a full length cDNA of Brassica. napus MKS1 （BnMKS1） was isolated from the line Zhongshuang 9 by homology-based cloning. BnMKS1 is a 217-amino-acid protein exhibiting an 82% similarity with AtMKS1. Sequence analysis showed that BnMKS1 contains a VQ domain and 8 potential MAP kinase phosphorylation sites. Agrobacterium-mediated infiltration transient assays in Nicotiana showed that BnMKS1 is localized in nuclei. Quantitative RT-PCR analysis revealed that the expression of BnMKS1 was strongly induced by Sclerotinia sclerotiorum, methyl jasmonate and benzothiadiazole. ABA did not affect the expression. It was suggested that BnMKS1 plays a role in the oilseed rape defense response against S. sclerotiorum. The results will offer useful information in further in-depth functional analysis of BnMKS1 in the interaction of oilseed rape and S. sclerotio- rum.