通过深层发酵法,对比研究了连续、分批、补料分批3种培养方式对樟芝真菌菌体,胞内外多糖产量的影响,研究表明,补料分批相对于连续培养,菌体最大产量提高5.51%,胞内多糖最大产量提高11.25%。胞外多糖最大产量提高21.70%,补料分批培养优于连续培养和分批培养。
The effects of continuous fermentation,batch fermentation and fed-batch fermentation on mycelium yield,intracellular and extracellular polysaccharide yields of Antrodia camphorata were studied by submerged fermentation.The results indicated that in comparison to continuous culture,the mycelium yield increased 5.51%,the yield of intracellular polysaccharide increased 11.25%,and the yield of extracellular polysaccharide increased 21.70% by fed-batch fermentation.Besides,the fed-batch fermentation was superior to continuous and batch fermentation.