中文摘要：

采用微加工方法在硅表面成功地制备出壳聚糖／牛血清白蛋白（CS／BSA）复合微图形．借助光镜和荧光显微镜对复合微图形进行形貌分析．利用大肠杆菌和白色葡萄球菌定量考察了CS／BSA复合微图形的抗菌性能，结果表明，硅表面沟槽交叉状CS／BSA复合微图形对两种细菌都具有较好的抗菌效果．通过MC3T3一E成骨细胞培养考察了复合微图形的细胞相容性，结果表明，CS／BSA复合微图形对于细胞的生长方向具有较强的诱导性，可促进细胞在材料表面的黏附、铺展及增殖分化．结果表明，采用微转移模塑法制备的CS／BSA复合微图形具有较好的抗菌性和细胞相容性．

英文摘要：

Chitosan/Bovine serum albumin （CS/BSA） composite micropatterns were successfully fabricated on silicon surfaces by micro-transfer molding method. The micropatterned CS/BSA composite films were stu- died by optical microscopy and fluorescent microscopy. A quantitative test was carried out to characterize the antibacterial properties of the CS/BSA composite films. The results show that the micropatterned CS/BSA composite films are effectively against E. coli and Staphylococcus albus. MC3T3-E cell culture test indicated that micropatterned CS/BSA composite films could strongly direct the orientation of cells. Moreover, the micropatterned CS/BSA composite films-could promote the cell adhesion, spreading, proliferation and differentiation. Thus, the micropatterned CS/BSA composite film fabricated by micro-transfer molding method has good antibacterial properties and cell compatibility.