中文摘要：

探讨了核苷类化合物指纹图谱用于不同海洋动物药真伪鉴别的可行性,为贵重动物药的鉴别提供了一种新方法.采用亲水色谱-电喷雾飞行时间质谱（HILIC-ESI-TOF/MS）对不同海洋动物药中的16种核苷类化合物进行分析,构建了基于16种核苷类化合物的特征指纹图谱,结合相似度分析和聚类分析,用于不同海洋动物药的鉴别.结果表明,基于核苷类化合物HILIC-ESI-TOF/MS分析的指纹图谱能反映不同海洋动物药各自的固有特征,结合相似度分析和聚类分析可实现对不同海洋动物药的正确区分.说明核苷类化合物指纹图谱有望成为动物药鉴别的新方法.

英文摘要：

The feasibility of identification of the authenticity for various marine animal drugs was investigated by a fingerprinting method based on the distribution of nucleoside compounds,and a new method for the discrimination of precious animal drugs was provided.Sixteen nucleoside compounds in various marine animal drugs were determined by hydrophilic interaction liguid chromatography-electrospray ionization-time of flight-mass spectrometry（HILIC-ESI-TOF/MS）.Characteristic fingerprint based on the distribution of 16 nucleosides was constructed,and it was used for identification of the authenticity of various marine animal drugs combined with similarity analysis and clustering analysis.The results indicated that the proposed characteristic fingerprint could reflect the inherent characteristics of their own for various marine animal drugs.Furthermore,it could be used to distinguish different marine animal drugs combined with similarity analysis and clustering analysis.In conclusion,the developed fingerprinting method is expected to be a powerful tool for the identification of animal drugs.