中文摘要：

目的：探讨CD4＋CD25＋调节性T细胞（即CD4＋CD25＋Treg细胞）在卵巢早衰发病机制中的作用。方法：流式细胞仪定量检测卵巢早衰（premature ovarian failure，POF）患者、卵巢储备功能下降（diminished ovarian reserve，DOR）患者及健康对照组外周血CD4＋T、CD8＋T细胞及CD4＋CD25＋Treg细胞数量，应用^3H-thymidine掺入法测定POF患者及对照组外周血CD4＋CD25＋Treg细胞对效应性T细胞的增殖抑制功能。结果：与对照组相比，POF患者及DOR患者CD4＋CD25＋Treg细胞比例降低（P〈0．01）、POF患者CD4＋T／CD8＋T细胞比值增高（P〈0．05）。DOR患者CD4＋T／CD8＋T细胞比值无明显变化（P〉0．05）；POF患者免疫抑制功能无明显降低（P〉0．05）。结论：CD4＋CD25＋Treg细胞比例降低与T细胞亚群失衡可能是POF的发病机制。

英文摘要：

Objective： To explore the role of T lymphocyte subsets and CD4＋CD25＋T regulatory cells （CD4＋CD25＋Treg cells） in the pathogenesis of premature ovary failure （POF）. Methods： Flow-cytometry analysis of CD4＋T, CDS＋T cells and CD4＋CD25＋Treg cells was performed on fresh peripheral blood in patients with POF （n=26）, diminished ovarian reserve （DOR, n=19） and control group, the suppression function of CD4＋CD25＋Treg cells in POF group and the control were detected by 3H-thymidine experiment. Results： Compared with the control, the percentages of CD4＋CD25＋Treg cells in POF group and DOR gruop were obviously reduced （P〈0.01）, and the CD4＋T/CDS＋T ratio in POF group was significantly in- creased （P〈0.05）; but in DOR group, there was no significant difference; however the suppression func- tion of CD4＋CD25＋Treg cells in POF group was not significantly decreased （P〉0.05）. Conclusion： The decreased CD4＋CD25＋Treg ceils and the imbalance of T lymphocyte subsets may play an important role in the pathogenesis of POF.