中文摘要：

目的：通过β受体阻滞剂普萘洛尔作用于小鼠EOMA血管瘤细胞体外增殖及凋亡的实验研究，初步探讨普萘洛尔治疗血管瘤的机制。方法：体外培养EOMA细胞，使用不同浓度普萘洛尔分别作用于EOMA细胞24、36、48h，应用噻唑蓝（MTT）法检测细胞存活率、吖啶橙染色检测细胞凋亡情况，观察普萘洛尔对EOMA细胞体外增殖和凋亡的影响。结果：作用24h后，随剂量增加，EOMA细胞存活率逐渐下降，与对照组相比，至药物浓度为75μmol／L时有显著差异（P〈0．05），继续增加至800μmol／L时，细胞存活率接近0，同时吖啶橙染色示凋亡细胞逐渐增多，与对照组相比，至药物浓度75μmol／L时，细胞凋亡率有显著差异（P〈0．05）。36h组和48h组变化趋势与24h组相似。结论：普萘洛尔在体外可有效抑制小鼠EOMA血管瘤细胞的增殖并促进其凋亡，这一作用呈现明显的剂量一时间效应依赖性。

英文摘要：

Objective： To explore the primary mechanism ofpropranolol treatment on hemangioma. Methods： Mouse hemangio- endothelioma endothelial （ EOMA ） cells cultured in vitro were used as the cell models in our study. Cells were treated using proprano- lol at different concentrations （ 5 μmol/L to 800 μmol/L ） for 24 h to 48 h. Cell proliferation was analyzed by MTT assay, and apoptosis was studied by acridine orange （AO） staining. Results： After 24 h to 48 h treatment, significant differences of cell viability and apopto- sis were noted （ P 〈 0.05 ） at the concentration of 75 μmol/L compared with the control group （ 0 μmol/L ）. When the dose was in- creased, cell viability dropped, whereas apoptosis dramatically increased. Conclusion： Propranolol can effectively inhibit the prolifera- tion and induce the EOMA cell anot）tosis in vltrn