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新疆野生山楂资源遗传多样性及亲缘关系的ISSR分析
  • ISSN号:1003-8981
  • 期刊名称:《经济林研究》
  • 时间:0
  • 分类:S661.5[农业科学—果树学;农业科学—园艺学]
  • 作者机构:新疆农业大学新疆特色果树研究中心,新疆乌鲁木齐830052
  • 相关基金:国家自然科学基金项目(30660122); 新疆维吾尔自治区果树重点学科资助项目
中文摘要:

为给新疆野生山楂资源的保护与利用提供理论依据和技术支持,采取新疆3种野生山楂共计63份材料,利用ISSR标记技术,对其遗传多样性和亲缘关系进行分析。结果表明,12条引物共扩增出233条带,其中多态性条带221条,多态性位点率为94.4%,平均每个引物获得19.42个扩增带。POPGENE1.32分析结果表明:3种野生山楂材料的多态性位点率、观测等位基因数、有效等位基因数、Nei’s基因多样性和Shannon信息指数,均为准噶尔山楂最高,阿尔泰山楂次之,红果山楂最低。3种新疆野生山楂进行两两比较,其Nei’s遗传相似度为0.862 2~0.904 4,平均值为0.890 2;遗传距离为0.100 5~0.148 3,平均值为0.1166。3种野生山楂中,准噶尔山楂存在较为丰富的遗传变异,其次是阿尔泰山楂,红果山楂的遗传变异较小。阿尔泰山楂和红果山楂的亲缘关系较近;准噶尔山楂和红果山楂间亲缘关系较远。

英文摘要:

In order to provide some theoretical basis and technical support for protection and utilization of wild hawthorn resources, genetic diversity and genetic relationship of 63 samples from three varieties of wild hawthorn resources in Xinjiang were analyzed by ISSR molecular marker technology. The results showed that 233 bands were amplified with 12 primers by the ISSR-PCR amplification, in which had 221 polymorphism bands, percent of polymorphism loci was 94.4%, and average 19.42 polymorphism bands were amplified by every primer. The results of analysis by the software of POPGENE1.32 showed that percentage of polymorphic loci, observed number of alleles, effective number of alleles, Nei's genetic diversity and Shannon information index of Junggar hawthorn were higher than other two kinds of wild hawthorn, and the indexes of Crabapple hawthorn were lower than Junggar hawthorn and Altai hawthorn. The Nei's genetic identities of three kinds of wild hawthorn were 0.862 2-0.904 4, and the average value was 0.890 2. The genetic distances of three kinds of wild hawthorn were 0.100 5-0.148 3, and the average value was 0.116 6. In the three kinds of wild hawthorn, genetic variation of Junggar hawthorn were more rich than the others, followed by AItai hawthorn, and Crabapple hawthorn had the less genetic variation. The genetic relationship between Altai hawthorn and Crabapple hawthorn was closer, but that was farther between Crabapple hawthorn and Junggar hawthorn.

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期刊信息
  • 《经济林研究》
  • 北大核心期刊(2011版)
  • 主管单位:湖南省教育厅
  • 主办单位:中南林业科技大学
  • 主编:谭晓风
  • 地址:湖南省长沙市韶山南路498号中南林业科技大学期刊社
  • 邮编:410004
  • 邮箱:jjlyj-csfu@163.com
  • 电话:0731-5658526
  • 国际标准刊号:ISSN:1003-8981
  • 国内统一刊号:ISSN:43-1117/S
  • 邮发代号:
  • 获奖情况:
  • 国内外数据库收录:
  • 中国中国科技核心期刊,中国北大核心期刊(2011版),中国北大核心期刊(2014版)
  • 被引量:11076