中文摘要：

本研究主要探讨毛叶假鹰爪素C（Desmosdumotin C）A环衍生物TEP诱导人急性白血病HL-60细胞凋亡作用及机制。流式细胞技术检测TEP诱导细胞凋亡及其对凋亡细胞中Fas、Fas L、Bax、Bcl-2表达率的影响,透射电镜观察凋亡细胞形态学改变。结果显示40μg/m L TEP作用细胞24 h后,细胞可呈现典型的凋亡形态学变化;40μg/m L TEP可明显提高凋亡细胞中Fas、Fas L、Bax的表达（P〈0.05）,并可明显降低抗凋亡细胞Bcl-2的表达（P〈0.05）。以上实验结果表明毛叶假鹰爪素C（Desmosdumotin C,Des C）A环衍生物TEP可有效地诱导HL-60细胞凋亡,其作用机制可能与上调Fas、Fas L、Bax表达以及下调Bcl-2表达有关。

英文摘要：

This study focused on the effects of TEP,an A ring derivative of desmosdumotin-C,on the human acute leukemia HL-60 cell apoptosis,and the underlying mechanisms. TEP-induced apoptosis and its effect on Fas,Fas L,Bax,Bcl-2expression were measured by flow cytometry,and the morphological changes of the apoptosis cells were further observed by transmission electron microscop. The results showed that 24 h after 40 μg / m L TEP treatment,the typical apoptotic morphological changes were induced in HL-60 cell. Meantime,40 μg / m L TEP significantly increased Fas,Fas L,and Bax expression（ P〈 0. 05）,and decreased Bcl-2 expression（ P 〈0. 05）. All the results suggested that TEP effectively induced apoptosis in HL-60 cells,and the mechanism may be involved in upregulating Fas,Fas L,Bax expression,and reducing Bcl-2 expression.