中文摘要：

目的：研究方便稳定的鼻灌洗方法,初步建立正常人鼻灌洗细胞学分类的正常值范围。方法：300例正常人按年龄分成3组,每组100例（男女各50例）,均为非吸烟的自愿者。用生理盐水进行鼻灌洗,并对鼻灌洗液中各种炎症细胞分类进行检测和统计学分析。结果：281例正常人鼻灌洗成功,成功率为93.67%。细胞学分类计数结果：嗜酸粒细胞（Eos）0.46±1.03、中性粒细胞4.46±9.84、淋巴细胞0.19±0.73和巨噬细胞0.04±0.16。对男女两组的各种细胞学参数采用独立样本t检验,组间比较差异无统计学意义（P〉0.05）。鼻灌洗Eos和中性粒细胞95%上限值分别为2.58和19.76。结论：本研究建立了操作方便、结果稳定的鼻灌洗方法,确定了鼻灌洗细胞学分类计数的正常值参考范围,其中Eos正常值为2.58。该鼻灌洗方法以及正常值的建立在研究鼻部炎症的特征、探讨鼻与下气道炎症的一致性规律、鼻炎患者的病情和疗效评估方面具有重要意义。

英文摘要：

Objective：To study the reference values for differential cell counts in nasal lavage of normality in Nanjing.Method：The total and differential cell counts were examined in nasal lavage from samples of a total of 300 healthy non-smoking adult volunteers.Result：Nasal lavage succeeded in 281 subjects,the achievement ratio was 93.67%.The proportions of eosinophils 0.46±1.03,neutrophils were 4.46±9.84,macrophages 0.19±0.73 and lymphocytes 0.04±0.16,respectively.There were no significant differences in the differential cell counts between male and female（P0.05）.The 95% ceiling percentile of eosinophils and neutrophils in nasal lavage are 2.58 and 19.76 respectively.Conclusion：The reference values were established for differential cell counts in nasal lavage of normality in Nanjing.We propose that these data be used in research on pathogenesis,diagnosis and treatment of the patients with nasal inflammation.