中文摘要：

为了挖掘光皮桦材性相关基因，本研究以光皮桦木质部为材料，通过对RNA的提取、cDNA的反转录及纯化的对比分析，以及选扩体系优化等，建立了相应的cDNA-AFLP体系。结果表明：采用CTAB和RNAiso试剂相结合的方法得到的RNA质量较好，cDNA经纯化后选扩效果明显变好。选扩体系为：4．0μL预扩产物（稀释40倍）、1．6μL的引物（10．0μmol／L）、0．2μL的dNTP（2．5mmol／L）、0．4μL的Mg2＋（25．0mmol／L），以及0．2μL的TaqDNA聚合酶（5U／μL）。进一步以来自不同木质化茎段的cDNA为模板，筛选出31对扩增效果较好的引物组合，同时分离出一些差异表达的基因片段，经测序后探讨了其在木质化发育过程中可能的功能。

英文摘要：

To investigate genes related to wood property in Betula luminifera, a suitable cDNA-AFLP reaction system for xylem tissues of B. luminifera was found in this study based on the comparative analysis of main procedures, including RNA extraction, reverse transcription and purification, and optimizing of selective amplification system. The results showed that CTAB method in combination with the RNAiso reagent was appropriate for RNA isolation of xylem, and cDNA purifying was an essential step after its synthesis. The optimal conditions for selective amplification were obtained as follows： the amount of 4.0 μL of 40 times-diluted preamplification products, 1.6μL of primer（ 10.0μmol/L） , 0.2μL of dNTP （2.5mmol/L）, 0.4 μL of Mg2＋ （25.0 mmol/L）, and 0.2 μL of Taq polymerase（5 U/μL）. In addition, 31 out of 64 primer pairs were screened by application of different lignification tissues in the selective amplification. Differentially expressed transcripts during the lignifieation were identified, and their function was analysed.