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松枯梢病菌RAPD分子水平遗传距离分析
  • 期刊名称:南京林业大学学报
  • 时间:0
  • 作者或编辑:3448
  • 页码:2006,30(1):13-16
  • 语言:中文
  • 分类:S763.122[农业科学—森林保护学;农业科学—林学] S791.22[农业科学—林木遗传育种;农业科学—林学]
  • 作者机构:[1]南京林业大学森林资源与环境学院,江苏南京210037, [2]江苏省林业局,江苏南京210036
  • 相关基金:国家自然科学基金资助项目(30070621)
  • 相关项目:松枯梢病菌群体遗传变异规律研究
中文摘要:

对来自中国、美国、英国、南非和智利的松枯梢病菌23个菌株进行RAPD分析。用12个引物共扩增出135个RAPD标记,其中多态性标记占96.3%。各菌株间的Nei相似系数UPGMA法聚类结果表明,国内外23个菌株可大致分为3个类群:来自智利的CWS41与所有菌株的遗传关系最远;来自中国的F2和J2次之;其他的国外菌株与国内菌株的遗传关系则较近。此次实验未将来自美国的B型菌株与其他菌株区分开。

英文摘要:

Genetic relationships were studied among 23 isolates of Sphaeropsis sapinea collected from China, United States, England, South Africa and Chile by using RAPD analysis method. 135 scorable DNA fragments were amplified with twelve random primers by PCR (Polymerase Chain Reaction) technique and the polymorphic score was 96. 3%. The genetic dendrogram based on RAPD analysis showed that the all S. sapinea isolates could be clustered into three groups. The isolate CWS41 from Chile was separated genetically as a first group away from other isolates, and isolates F2 and J2 from China were as the second group. The third RAPD group accommodated other rest isolates (including B morphotype CWS43 from United States) .

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