中文摘要：

以普定严重石漠化区域石生细叶小羽藓Haplocladium microphyllum Hedw.、银叶真藓Bryum argenteum Hedw.和美灰藓Eurohypnum leptothollum（C.Muell.）Ando.为研究材料,运用模拟土柱实验装置收集植物淋出液并测定其电导率、pH值、CA活性、钙镁元素含量并对石灰岩试片溶蚀进行估算。结果表明：岩溶生态系统中不同植物的碳酸酐酶活性差异较大,其中细叶小羽藓的溶蚀效果最为显著,产生的碳汇量最大;CA活性最高为美灰藓,为272.99U·g~（-1）·FW;最小的为细叶小羽藓的CA活性较低,为38.31U·g~（-1）·FW。淋出液均检测到CA的存在,银叶真藓淋溶6d的CA活性最高,随着时间的推移,3种植物CA总体表现出先略降,然后升高,最后趋于平稳的趋势;pH值呈上升趋势;电导率呈下降趋势;淋出液中Ca^2＋和Mg^2＋浓度随淋溶时间的延长浓度逐渐降低,一定时间后趋于动态的平衡;说明石生植物对石灰岩有明显的酶促溶蚀驱动作用,且溶蚀率与CA活性成正相关。

英文摘要：

Bryophytes play an important role in forest ecosystems,which are irreplaceable to soil and water conservation and the improvement of the ecological environment.Due to the ecological environment deterioration of rocky desertification in Guizhou province,serious soil erosion problem have been caused,which seriously affect the production and living of the masses.So,it is necessary to strengthen the restoration and management of the ecological environment in the region.This work took three epilithic mosses,the H.microphyllum,B.argenteum,E.leptothollum,as research materials.Using the device of simulated soil column experiment,we collected leachate and measured the carbonic anhydrase（CA）activity,electrical conductivity,pH,the content of calcium and magnesium elements and estimated the effect to limestone specimens.The results show that there are some differences about carbonic anhydrase activity of these epilithic mosses.The E.leptothollum is highest which reaches to 272.99 U·g-1.FW,whereas the minimum is 38.31U·g-1.FW in H.microphyllum.All leachate was detected the existence of CA activity,and the leachate of B.argenteum 6 denzyme solution has the highest activity.In all,the CA activity in the 3 kinds of plants shows a tendency to decrease slightly fisrt,then increases,and finally tends to be stable.Their pH values exhibit a rising trend while electrical conductivity has downturn.The concentrations of Ca2＋and Mg2＋gradually drop with the extension of leaching time,and then tend to dynamic equilibrium after a certain time,which shows that the lithophyte has a significant enzymatic driving effect on limestone and there is a positive correlation between the dissolution rate and CA activity.This work is of great academic significance to further understanding the dynamics of CA activity produced by the metabolism of stone bryophytes in karst areas and their erosion to limestone.