中文摘要：

目的观察脑缺血后处理中，联合水蛭注射液进行后处理，对脑缺血损伤大鼠磷酸化蛋白激酶BmRNA及其蛋白表达的影响。方法SD大鼠40只，随机分为假手术组（s0组）、缺血再灌注组（I／R组）、缺血后处理组（IPOC组）、IPOC＋水蛭注射液后处理组（P-L组），每组10只。采用线栓法制备大鼠大脑中动脉闭塞模型及IP0c模型，应用实时荧光定量PCR和Westernblot法检测磷酸化蛋白激酶mRNA及其蛋白表达。结果s0组有少量磷酸化蛋白激酶mRNA及蛋白表达；与SO组比较，I／R组表达明显升高；与I／R组比较，IPOC组表达明显升高（P％0．01）；与IPOC组比较，P—L组表达明显升高（P〈0．05）。结论IPOC可能通过诱导磷酸化蛋白激酶的表达发挥其神经保护作用，水蛭注射液可进一步促进其表达而发挥神经保护作用。

英文摘要：

Objective To study the effect of combined leech injection and ischemic postconditioning on expression of p-Akt mRNA and protein in rats following I/R injury. Methods Forty male SD rats were randomly divided into sham operation group, I/R group, ischemie posteonditioning group and leech injection postconditioning group （10 in each group）. A middle cerebral artery oc- clusion model and an isehemie posteonditioning model were established by Longa occlusion. Ex- pression of p-Akt mRNA and protein was detected by RT-PCR and Western blot, respectively. Results Only a mall amount of p-Akt mRNA and protein was expressed in sham operation group. The expression level of p-Akt mRNA and protein was significantly higher in I/R group than in sham operation group,in ischemie postconditioning group than in I/R group, and in leech injection postconditioning group than in ischemie postconditioning group （P〈0.05）. Conclusion Ischemic posteonditioning can protect nerves by inducing p-Akt expression and leech injection postconditioning plays an important role in protection of nerves by further promoting its expression.