中文摘要：

TP53基因在调控细胞信号通路和抑制肿瘤细胞中发挥着重要作用.本研究利用毕赤酵母表达系统以获得大量重组p53蛋白,为此,本文将人TP53基因克隆至表达载体p PIC3.5K,电转化进入毕赤酵母GS115,通过组氨酸筛选和G418筛选,获得高拷贝稳定转化子.SDS-PAGE和Western Blot检测结果表明p53蛋白可以在毕赤酵母中表达.本研究所获酵母菌株为今后大规模生产重组p53蛋白奠定了基础.

英文摘要：

TP53 gene plays a crucial role in the inhabitation of tumor progression and regulation of signal transduction. In this study, abundant recombinant p53 protein was harvested by using the Pichia pastoris expression system. To achieve this aim, human TP53 gene was inserted into the expression vector p PIC3.5K, and transformed to Pichia Pastoris GS115 by electroporation.Stable high copy number TP53 transformants were obtained by screening with histadine and G418. SDS-PAGE analysis and Western Blot demonstrated that p53 protein was expressed in Pichia pastoris. The strains obtained in this study established a firm foundation for large scale production of recombinant p53 protein in the future.