中文摘要：

为获得适合转化百合的双价RNAi表达载体,以感病百合叶片的总RNA为模板,分别扩增400bp左右的LSV和LMoVCP基因。通过重叠延伸PCR技术获得长约800bp的LSV-LMoV融合基因。利用Gateway技术,通过BP反应及LR反应将LSV-LMoV融合基因克隆到双元载体pH7GWIWG2（Ⅱ）,成功构建了含2种病毒基因的RNAi表达载体pH7GWIWG2（Ⅱ）-LSV-LMoV。将构建好的双价表达载体导入农杆菌EHA105,PCR和测序表明所构建的载体及获得的工程菌与预期的完全一致。

英文摘要：

In order to gain the RNA interference（RNAi） transformation vector of lily,two fragment genes about400 bp of LSV and LMoV were obtained from the total RNA of lily leaves infected.LSV-LMoV fusion gene was constructed by recombinant PCR technique.Using Gateway cloning technology,the RNAi transformation vector pH7GWIWG2（Ⅱ）was constructed by BP and LR recombination reactions.PCR and sequencing analysis confirmed that the pH7GWIWG2（Ⅱ）-LSV-LMoV vector,a binary vector containing two different virus genes,was obtained successfully.Virus susceptible lily was transformed with pH7GWIWG2（Ⅱ）-LSV-LMoV by Agrobacterium tumefaciens-mediated transformation.