中文摘要：

目的：建立人卵巢上皮性癌荷瘤裸鼠不良心理应激模型，研究不良心理应激对荷瘤裸鼠肿瘤生长的影响。方法：首先采用瘤组织块接种法，建立人卵巢上皮性癌裸鼠皮下移植瘤模型，待肿瘤生长至接种后第12天，将肿瘤大小相近（约8mm）的12只裸鼠随机分为：荷瘤组和荷瘤＋应激组，每组6只；荷瘤组自由供食物和水。荷瘤＋应激组采用束缚方法给予应激，装置为50mL离心管（打孔3mm，通风良好），操作时依次将应激组小鼠放入离心管中。不挤压动物尾巴，放入管内动物可前后活动。8h后将动物放出，恢复自由供食物和水，每天10：00AM～6：00PM，应激5d／周，共2周；最后观察两组裸鼠生存状态、体重，完成2周应激，结束后第二天，两组裸鼠同时眼眶取血，离心后吸取血清，采用酶联免疫吸附法（ELISA方法）检测两组裸鼠血清中皮质酮、去甲肾上腺素的含量。结果：1）荷瘤＋应激组整体生长状态不良，进食及活动不佳，荷瘤＋应激组裸鼠体重明显低于荷瘤组，两组比较，差异有显著性（P〈0．05），不良心理应激使荷瘤裸鼠体重明显降低；2）荷瘤＋应激组血清中皮质酮、去甲肾上腺素的含量明显高于荷瘤组，两组比较，差异有显著性（P〈0．05）。结论：采用束缚方法成功建立了人卵巢癌荷瘤裸鼠不良心理应激模型，该模型可用于研究不良心理应激对卵巢癌生长的影响。

英文摘要：

Objective： To establish dys-psychological stress model of human epithelial ovarian carcinoma in nude mice for the observation of adverse psychological stress on tumor growth. Methods： The subcutaneous tumor xenografts were established by implanting tumor tissues into the nude mice. Twelve clays later, when the subcutaneous tumors were found about 0.8 cm in diameter, the mice were randomly divided into the following two groups （n=6/group） respectively： the dys-psychological stress model group and the control group. The dys-psychological stress model was established by putting each nude mouse respectively into a 50mL centrifuge tube with a 3mm hole for excellent ventilation. The mice were not squeezed their tails and could move in the tubes. Eight hours later, the animals were released from the tubes and then supplied with food and water. During the study the mice of dys-psychological stress model were restrained 8h/day, 5d/week for two weeks. At 2 days after the restraint course, the mice were weighed, and the blood samples were collected. ELISA was used to detect corticosterone （CORT） and norepinephrine （NE） in serum. Results： The mice in the stress group were in a poor state of growth, food intaking and action, and the average of mice weight in the dys-psychological stress group was significantly decreased compared with the mice in the control group （P〈0.05）. But the levels of CORT and NE in the serum of mice in the stress group were higher than those in the control group （P〈0.05）. Conclusion： The dys-psychological stress model of human epithelial ovarian carcinoma in nude mice was successfully established, and it can be utilized for research of the effect of psychological stress on ovarian carcinoma cell growth.