中文摘要：

近年来DNA条形码的广泛应用推动了包括分类学在内的多学科的发展,促进了进化生物学及生态学的研究,受到高度关注。DNA条形码不仅能够简单地用于物种识别,还可以用于动物食性、食物链及食物网的研究,主要通过提取粪便及肠道内容物的短片段DNA来得到实现。本文阐述了DNA条形码的研究概况及其在植食性昆虫食性鉴定中的应用,并详细介绍了用于植食性昆虫食性鉴定的DNA条形码的选择,最后针对鳞翅目幼虫食性相关研究予以综述。植食性鳞翅目幼虫对农林业有着严重的为害,其营养关系的研究对有害生物防治具有重要意义。

英文摘要：

DNA barcoding has been widely used and received much attention in recent years,pushing the development of multidisciplinary researches including taxonomy,and promoting the studies of evolutionary biology and ecology. DNA barcoding is not only used in initially proposed simple species identification,but also in studies of animal diets,food chain,and food web by using short DNA fragments extracted from in feces and intestinal contents of animals. In this article,we systematically reviewed the recent advances in DNA barcoding and its application in the identification of diets of insect herbivores,and elaborated the selection of DNA barcodes in the identification of herbivore diets and the contribution in the diet composition analysis. Finally,the related research of diets of Lepidoptera larvae was reviewed.Since the herbivorous Lepidoptera larvae cause serious damages and losses to forestry and agriculture,the study of their trophic relationship has great significance for pest control and management.