中文摘要：

为研究集胞藻6803中两个S2P同源蛋白酶Slr0643和Sll0862的功能,分别在两个蛋白酶的羧基端添加增强型绿色荧光蛋白（EGFP）标签,构建了两株转基因集胞藻psbA2：：0643GFP-Cmr/△0643-Kmr和psbA2：：0862GFP-Cmr/△0862-Kmr.通过逆转录聚合酶链式反应（RT-PCR）检测到egfp基因的转录表达,通过激光共聚焦显微镜检测到转基因集胞藻细胞发出的绿色荧光蛋白荧光,表明带EGFP标签的S2P融合蛋白在psbA2启动子调控下正确表达.

英文摘要：

In order to explore the functions of site-2 protease （S2P） homologs Slr0643 and Sl10862 in Synechocystis sp. PCC 6803, enhaneed green fluorescent protein （EGFP） was added to the earboxyl terminal as a protein tag, and two transgenie Syneehoeystis sp. PCC 6803, namely, psbA2：： 0643GFP-Cmr/△0643-Km^t and psbA2：： 0862GFP-Cmr/△0862-Km^r, were obtained. The transcriptional expression of egfp in two transgenic lines was then deteeted via RT-PCR, and the GFP fluorescenee was observed by using a laser scanning confocal mieroseope. Both of these confirm the sueeessful expression of S2P fusion proteins tagged with EGFP under the psbA2 promoter.