中文摘要：

目的：优化RNA甲醛琼脂糖凝胶电泳条件。方法：对实验器材进行彻底的RNase灭活处理;在原有电泳过程的基础上,增加预电泳、缓冲液液面的处理和制胶过程中未加入溴化乙锭等优化过程。结果：优化后的电泳不仅能验证总RNA的完整性,而且能鉴定RNA的分子大小。结论：优化的RNA甲醛琼脂糖凝胶电泳法操作简单、省时、快速,RNA条带清晰、定位准确、无弥散及非特异条带。

英文摘要：

Objective：To opitimize the conditions of the RNA formaldehyde agarose gel electrophoresis.Methods：Thoroughly in-activate RNase of experimental equipments;Based on the original electrophoresis process,adding the opitimized procedures,such as：pre-electrophoresis,treatment of buffer surface and decreasing ethidium bromide into gel etc.Results：The opitimized RNA electrophoresis not only verify the integrity of total RNA,but also identify the molecular size of RNA.Conclusion：The opitimized RNA electrophoresis is simple,time-saving,fast and RNA bands are clear,accurate position,no dispersion and non-specific bands.